The present study applied the comet assay to erythrocytes of Oreochromis niloticus with the aim of improving protocols to detect DNA damage in these cells, by using two distinct pHs (pH = 12.1 and pH > 13) and evaluating whether there is a correspondence between silver and ethidium bromide staining. Comets were visually examined and, the frequency of cells with and without damage was obtained, ...

Howard, Dexter H. (University of California, Los Angeles). Intracellular behavior of Histoplasma capsulatum. J. Bacteriol. 87:33-38. 1964.-The tinctorial characteristics and intracellular generation time of Histoplasma capsulatum within mouse histiocytes maintained in tissue culture were studied. The appearance of viable and nonviable cells of H. capsulatum was observed after staining with the ...

Physical mapping of the 18S ribosomal RNA gene (rDNA) was carried out by fluorescent in situ hybridization (FISH) in the endangered freshwater fish O. belangeri. The specimens were collected from Imphal valley, Manipur, India and metaphase chromosome preparation was made using standard hypotonic treatment, methanol-acetic acid fixation and flame-drying technique followed by Giemsa, silver, CMA3...

The variations in the numbers of nucleolar organising regions (NORs) among different grades of cervical intraepithelial neoplasia (CIN) were investigated using a silver staining technique. Twenty four biopsy specimens were studied (six normal and six of each of the three grades of CIN) by staining paraffin wax sections using a silver (AgNOR) method that stains the NORS as multiple black dots wi...

Active nucleolar organizer regions (NORs) are affine to silver (Ag) and therefore visible in silver stained cell specimens. These so called AgNORs are of diagnostic relevance for early cancer diagnosis. Therefore we seek to automatically detect and segment these dark, spot-like regions. This is challenging, since the contrast between the AgNORs and the nucleus background varies heavily from cel...

Different methods for the isolation of soluble proteins were applied to cell cultures of three apple cultivars (Malus sylvestris Mill.), best results being obtained with a rapid technique based on freezing and thawing. Ultrathin-layer isoelectric focusing followed by an improved silver staining method has shown that proteins from apple callus cultures consist of some 60 to 80 zones, with isoele...

Measurement of telomerase activity in clinically obtained tumor samples may provide important information for use as both a diagnostic marker and a prognostic indicator for patient outcome. In order to evaluate telomerase activity in tumor tissue without radiolabeling the product, we developed a simple telomeric repeat amplification protocol-silver-staining assay that is less time-consuming, is...

A high-throughput optical biosensing technique is proposed and demonstrated. This hybrid technique combines optical transmission of nanoholes with colorimetric silver staining. The size and spacing of the nanoholes are chosen so that individual nanoholes can be independently resolved in massive parallel using an ordinary transmission optical microscope, and, in place of determining a spectral s...

Chromosome morphology was studied in lily genotypes L. candidum, L. × formolongi, L. henryi and L. pumilum, and cultivars ‘Alma Ata,’ ‘Expression,’ ‘Marco Polo,’ ‘Muscadet’ and ‘Star Gazer’ belonging to the horticultural group Oriental hybrids. All genotypes tested represented 2n = 2x = 24 chromosomes. Chromosomal markers were established after Feulgen and silver staining, from analysis of the ...

Background: Dental caries is the most frequent chronic disease worldwide. However, can be prevented or arrested. Recently interest in use of silver diamine fluoride (SDF) has been growing. a solution containing ionic silver, fluoride, and ammonia that arrests progress carious lesions prevents development future caries. In Vitro studies demonstrated SDF increases pH biofilm, reduces dentin demin...