This article shows the validity of Universal calibration concept in aqueous Size-exclusion chromatography for arabinogalactan and carboxymethylchitosans is presented. The electrostatic effects these polysaccharides during separation process were eliminated by utilizing a water-salt eluent as mobile phase, size-exclusion mechanism this method was realized.

Lentiviral vectors (LVs) have been successfully used in clinical trials showing long term therapeutic benefits. Studying the role of cellular proteins in lentivirus HIV-1 life cycle can help understand virus assembly and budding, leading to improvement of LV production for gene therapy. Lentiviral vectors were purified using size exclusion chromatography (SEC). The cellular protein composition ...

Size exclusion chromatography (SEC) is a widely used tool in bioseparations. Because its separation mechanism is based on the permeability of macromolecules rather than any type of binding, the feed volume to bed volume ratio is usually quite small. Thus, large columns are typically used in preparativeand large scale separations. In this work, a general rate model considering various mass trans...

PEGylation of therapeutic proteins has signifi cantly improved their value by altering the physicochemical and biological properties such as increased solubility, decreased immunogenicity, increased half-life, and protection against proteases. Size Exclusion Chromatography (SEC) is the method of choice for determining impurities with molecular mass higher than that of PEGylated proteins. SEC of...

Melanoma is the most aggressive skin cancer and responsible for majority of deaths. Despite advances in treatment, our current prognostic indicators are poor fail to adequately risk-stratify patients. This study evaluates concentration, size, proteomic metabolomic profile circulating extracellular vesicles (EVs) isolated from plasma patients with melanoma, identify potential biomarkers disease ...