The induction of ornithine decarboxylase (ODC) activity may be an essential component of skin tumor promotion. ODC requires pyridoxal 5'-phosphate (PLP) as a cofactor. We have measured the epidermal PLP concentration and investigated its relationship to DNA synthesis and ODC activity in the hairless mouse. The epidermal PLP concentration was approximately 1.0 microgram/g. When tape-stripping wa...

Cathodic adsorptive stripping voltammetry (CASV) was applied for the determination of rutin in pharmaceuticals, human urine, and blood serum. An electrochemical stripping procedure for trace measurements of rutin was developed based on the adsorption of the Cu2þ-rutin complex on a hanging mercury drop electrode and applied to the quantification of the drug. Cyclic voltammetry was used to charac...

Ceramides (CERs) in the upper layer of the skin, the stratum corneum (SC), play a key role in the skin barrier function. In human SC, the literature currently reports 11 CER subclasses that have been identified. In this paper, a novel quick and robust LC/MS method is presented that allows the separation and analysis of all known human SC CER subclasses using only limited sample preparation. Bes...

For the investigation of the skin irritancy potential of chemicals in an in vitro model, it is necessary to have sensitive end-points that predict the effects on native human skin. Our aim was to investigate whether the induction of the proteinase inhibitor SKALP in reconstructed epidermis can be used as a marker. The influence of culture conditions and the effect of topical application of sodi...

Aim: The objectives of this study were to investigate the effects of tooth extraction and air-rotor stripping therapy on tooth-size discrepancy, and to compare the changes between two groups. Materials and methods: The sample comprised the preand post-treatment dental models of 20 postadolescent class I borderline patients. First group was composed of dental models of 10 patients (mean age of 1...

BACKGROUND The aim of this study is to evaluate the change in pulp chamber temperature during the stripping technique. METHODS Seventy-eight proximal surfaces of 39 extracted human teeth were stripped by two techniques: double-sided perforated stripping disk (PSD) and handheld stripper (HS). The teeth were divided into three groups: incisors (group 1), premolars (2), and molars (3). A J type ...

We report here on the application of laser-based single molecule total internal reflection fluorescence microscopy (TIRFM) to study the penetration of molecules through the skin. Penetration of topically applied drug molecules is often observed to be limited by the size of the respective drug. However, the molecular mechanisms which govern the penetration of molecules through the outermost laye...

BACKGROUND Herpes simplex virus infection (HSV) is a common and ubiquitous infection of the skin which causes mucocutaneous lesions called cold sores (herpes labialis) or fever blisters. It is estimated that approximately 80% of the population worldwide are carriers of the Herpes simplex virus, approximately 40% suffer from recurrent recurrent infections. This study evaluates the in vitro skin ...

We have recently shown that mechanical forces, experimentally imposed or physiologically generated, transiently disrupt or wound the plasma membranes of epithelial cells of the gut and that cultured endothelial cells similarly wounded mechanically at their plasma membranes release a potent basic fibroblast growth factor-like molecule. Here we show that mechanical forces generated by experimenta...