A surface plasmon resonance imaging-based Ni(2+)-iminodiacetic acid-coated gold chip system was developed to enable specific detection of a hexahistidine-tagged recombinant protein in crude extracts or in column chromatography fractions. This system is especially advantageous for high-throughput analysis of multiple proteins.

Fluorescent probes are essential for the exploration of protein function, detection of molecular interactions, and conformational changes. The nitrilotriacetic acid derivatives of different chromophores were successfully used for site-selective noncovalent fluorescence labeling of histidine-tagged proteins. All of them, however, suffer from the same drawback--loss of the fluorescence upon bindi...

Affinity tags have become powerful tools from basic biological research to structural and functional proteomics. They were widely used to facilitate the purification and detection of proteins of interest, as well as the separation of protein complexes. Here, we mainly discuss the benefits and drawbacks of several affinity or epitope tags frequently used, including hexahistidine tag, FLAG tag, S...

The article reveals the specificity of heterotopic approach in analysis artistic texts and characterizes functions heterotopias modern Ukrainian drama. Examining dramatic as «other space» it was found that playwrights at liminal stage resorted to creating image a parental house, village, provincial town, which realize heterotopia function prison breaks time; Maidan, Donbass museum archive accum...

THE NOTION OF A Socratic PHILOSOPHY, as distinct from what is commonly known as Platonism, has its origins in the work of Gregory Vlastos1 and continues today through the scholars he has influenced.2 The Socratic movement in Plato studies maintains that one can identify in certain Platonic dialogues a philosophical method and a collection of philosophical theses which may properly be attributed...

A drama manager is an omniscient background agent responsible for guiding players through the story space and delivering an enjoyable and coherent experience. Most previous drama managers only consider the designer’s intent. We present a drama manager that uses data-driven techniques to model players and provides personalized guidance in the story space without removing player agency. In order ...

this study is the result of an attempt to investigate the differences between the persian translated drama text (page) of each english drama text with its performance on the stage (stage) in iran. in other words, the present researchers tried to find the implemented changes in page which make it real on the stage in the target language and culture in order to show that in drama translating and ...