Partial reduction of the basic pancreatic trypsln inhibitor with sodium borohydride results in the selective cleavage of the disulfide bond linking half-cystine residues 14 and 38. The partially reduced inhibitor is fully active, whereas its carboxymethylated derivative is completely inactive and is susceptible to tryptic digestion. The reduced inhibitor slowly reoxidizes when incubated alone a...

Very few nitrosyl derivatives of the first transition elements are known [1, 2], [^-C5HsCr(NO)2]2 has been obtained by reduction of ^-CsHsCrNOCl with sodium borohydride [3]. The only known cyclopentadienylnitrosyl vanadium complexes are T?-C5H5V(NO)2CO [4] and the cation [>?-C5H5V(NO)3]+ [5]. The most important methods used to obtain these complexes consist of treatment of carbonyl complexes wi...

Radioactivity incorporated into an e-N-5’-phosphopyridoxyllysine residue of the /3 chain by reduction of the flZ holoenzyme with tritiated sodium borohydride appears in two tryptic peptides. These pyridoxyl peptides differ by only a single arginyl residue. As expected, tryptic digests of normal protein contain two peptides that together make up the pyridoxyl peptide region. The primary structur...

Simple primary-tertiary diamines easily derived from natural primary amino acids were used to catalyze the Michael addition of ketones with isatylidenemalononitrile derivatives. Diamine 1a in combination with D-CSA as an additive provided Michael adducts in high yield (up to 94%) and excellent enantioselectivity (up to 99%). The catalyst 1a was successfully used to catalyze the three-component ...

Nanotechnology has great promise for improving the therapeutic potential of medicinal molecules and related agents. In this study, silver nanoparticles of different sizes were synthesized in an ultrasonic field using the chemical reduction method with sodium borohydride as a reducing agent. The size effect of silver nanoparticles on antimicrobial activity were tested against the microorganisms ...

Silver/polylactide nanocomposites (Ag/PLA-NCs) were synthesized via chemical reduction method in diphase solvent. Silver nitrate and sodium borohydride were used as a silver precursor and reducing agent in the polylactide (PLA). The properties of Ag/PLA-NCs were studied as a function of the weight percentages of silver nanoparticles (8, 16 and 32 wt% of Ag-NPs) relative to the weight of PLA. Th...

To ensure targeting of apoferritin nanocarrier with encapsulated doxorubicin drug, we used a peptide linker based on a protein G with N-terminus affinity towards Fc region of antibodies. To connect the peptide to the surface of apoferritin, the C-terminus of peptide was made of cysteine with affinity to gold. The surface of apoferritin with encapsulated doxorubicin (APODOX) was coated either wi...