Our objectives were to determine if milk casein as a percentage of true protein (CN%TP) estimated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) is equivalent CN%TP Kjeldahl, and the proportion (CN), proteolysis products (CNPP), serum (SP) from (TP) that goes into Kjeldahl noncasein nitrogen (NCN) filtrate stays in NCN precipitate using SDS-PAGE. Raw samples collected 1...

Surfactant-based foam delivery technology has been studied to remediate Hanford 200 area deep vadose zone sediment. However, the surfactants and remediation amendments have an unknown effect on indigenous subsurface microorganisms. Microbial populations are important factors to consider in remediation efforts due to their potential to alter soil geochemistry. This project focuses on measuring m...

the human papillomavirus l1 major capsid protein (hpv l1), the basis of the current vaccines, self-assembles into virus-like particles (vlps). herein, we describe the expression and purification of recombinant hpv16 l1 in e. coli system. the l1 protein was generated in a fused form using an inducible expression system. the recombinant gst-l1 fusion protein migrated as a 82 kda protein in sds-pa...

The properties of porous and lightweight ceramic foam that can be cured at room temperature using metakaolin-based geopolymers were studied. A geopolymer slurry was prepared metakaolin a potassium-based alkaline medium temperature, the obtained viscous paste expanded via gaseous methods, by means decomposition peroxide temperature. Therefore, (GP) developed in this study through multivariate ge...

Using nanopores for single-molecule sequencing of proteins - similar to nanopore-based sequencing of DNA - faces multiple challenges, including unfolding of the complex tertiary structure of the proteins and enforcing their unidirectional translocation through nanopores. Here, we combine molecular dynamics (MD) simulations with single-molecule experiments to investigate the utility of SDS (Sodi...

The binding of curcumin on the surface of AgNPs-sodium dodecyl sulfate (SDS)-multi-walled carbon nanotubes–glassy electrode (CM/AgNPs/SDS/MWCNTs) was reported for simultaneous electrochemical determination of dopamine (DA), uric acid (UA) and guanine (GU). Morphological properties modified electrodes surfaces were characterized using different voltammetry methods. The microscopic area the obtai...

BACKGROUND Prions demonstrate an unusual resistance to methods effective at inactivating conventional microorganisms. This has resulted in a very tangible and difficult infection control challenge to the medical and veterinary communities, as well as animal agriculture and related industries. Currently accepted practices of harsh chemical treatments such as prolonged exposure to sodium hydroxid...

A novel structural solid dispersion (SD) taking the form of core-shell microparticles for poorly water-soluble drugs is reported for the first time. Using polyvinylpyrrolidone (PVP) as a hydrophilic polymer matrix, the SDs were fabricated using coaxial electrospraying (characterized by an epoxy-coated concentric spray head), although the core fluids were unprocessable using one-fluid electrospr...

This experimental study aims to examine the partial discharge (PD) properties of palm oil and coconut (CO) based aluminum oxide (Al2O3) nanofluids with without surfactants. The type surfactant used in this was sodium dodecyl sulfate (SDS). volume concentrations range Al2O3 dispersed samples varied from 0.001% 0.05%. ratio surfactants nanoparticles set 1:2. In total, two different types refined,...