Two previously undescribed activators of prekallikrein have been purified from human plasma by alcohol frac tionation, ion exchange chromatography, gel filtration, and polyacrylamide gel electrophoresis. Both were apparently homogeneous as judged by electrophoretic mobility and molecular weight. A monomer-dimer relationship between these two activators is suggested by the use of disc gel electr...

The rewards of biomedical research can be very exciting and remarkable. The feeling of having done an experiment for the first time and only you know the results that will have an important effect on your research and perhaps broader applications is exhilarating. I suspect that it is not common for scientists to see their fundamental research develop during their research career into such broad...

Details are given of the selection and standardization of reagents for use in a standard clot method for estimating plasminogen activators, anti-activators, and plasmin.

Eukaryotic transcription activation domains (ADs) are not well defined on the proteome scale. We systematicallly tested approximately 6000 yeast proteins for transcriptional activity using a yeast one-hybrid system and identified 451 transcriptional activators. We then determined their transcription activation strength using fusions to the Gal4 DNA-binding domain and a His3 reporter gene which ...

For the first time in the present study the effects of vanillin, vanillyl alcohol, vanillic acid, as well as the newly synthesized vanillin derivative, bis-vanillin, were investigated on the oxidation of dopamine hydrochloride by mushroom tyrosinase. Among them, vanillin and bis-vanillin act as activators, while vanillyl alcohol and vanillic acid exhibited inhibitory effects, the IC50 values be...

Abstract Separation of seed mixtures for the purpose their purification or calibration by fractions is a mandatory technological operation in processing most crops. Due to its properties, process separation individual crops has undefined parameters low efficiency flow. A similar material safflower, which were determined work taking into account different methods components mixture. The involves...

Adenosine-to-inosine RNA editing modifies maturing mRNAs through the binding of adenosine deaminase acting on RNA (Adar) proteins to double-stranded RNA structures in a process critical for neuronal function. Editing levels at individual editing sites span a broad range and are mediated by both cis-acting elements (surrounding RNA sequence and secondary structure) and trans-acting factors. Here...

Several prothrombin activators from snake venoms have been isolated and characterized (For details, see 1). Initially, they were classified into three major groups based on their structural and functional properties (1, 2). They differ from each other in their cofactor requirements and the products formed. Group I activators are metalloproteinases. They convert prothrombin to meizothrombin and ...

Stereoregulated diribonucleoside phosphorothioates were synthesized by the use of 2'-O-TBDMS-protected ribonucleoside 3'-O-oxazaphospholidine derivatives as monomers and N-(cyanomethyl)ammonium salts as activators. Diastereoselectivity of the condensation reaction was found to be highly dependent on the substituent groups of the oxazaphospnolidine ring as well as the structure of the activators...

Metal-based bifunctional methane activators are modeled utilizing density functional theory. The impact upon activation barriers of the metal among late 3d MII ions (Co – Zn) is quite pronounced, ca. 7 kcal/mol. Alteration metal’s coordination environment without changing supporting ligand types (e.g., comparing cis and trans isomers) has a computed on ± 1 One significant result involves varyin...