Abstract Background Primary hepatic mucoepidermoid carcinoma (HMEC) is extremely rare and the molecular etiology still unknown. The CRTC1-MAML2 fusion gene was previously detected in a primary HMEC, which often associated with MEC of salivary gland literature. Methods A 64-year-old male diagnosed HMEC based on malignant squamous cells mucus-secreting immunohistochemical examination. Fluorescenc...

The regulated translation of localized mRNAs in neurons provides a mechanism for spatially restricting gene expression in a synapse-specific manner. To identify the population of mRNAs present in distal neuronal processes of rodent hippocampal neurons, we grew neurons on polycarbonate filters etched with 3 microm pores. Although the neuronal cell bodies remained on the top surface of the filter...

Some of the cells of every human being will grow outside the body as microorganisms. It is possible to show, in a variety of ways, that these cells resemble genetically the individual from whom they were obtained. Over 35 inherited human diseases and anomalies can now be studied in such cell lines. Human diploid cell strains, biochemically marked by one or more mutant Mendelian genes, have prov...

Monoclonal antibodies prepared by somatic cell hybridization techniques are ideal tools for the discrimination of cellular antigens and are beginning to reveal qualitative and quantitative differences in the antigenic composition of normal and malignant cells. Because these reagents are homogeneous in nature, recognize specific antigenic determinants, and can be mass produced, monoclonal antibo...

The relative efficiency of microcell-mediated chromosome transfer vs. somatic cell hybridization has been determined. The prolonged mitotic arrest generally used to micronucleate donor cells also reduced the fusion efficiency to 1/10th-1/5th of that in whole cell hybridizations. Here we report an alternative micronucleation protocol, involving sequential treatment of the donor cells with Colcem...

Paroxysmal nocturnal hemoglobinuria (PNH) is a hemolytic disorder caused by a deficiency of biosynthesis of the glycosyl phosphatidylinositol (GPI) anchor, but the biochemical defect is not completely understood. In the present study, we have analyzed affected cell lines established recently from two Japanese patients with PNH. Two lines of evidence indicate that these cells do not synthesize N...

Aryl hydrocarbon (benzo(a)pyrene) hydroxylase is present and inducible in Buffalo rat liver cells in culture. There is substantial variation in both basal and inducible hydroxylase activities among heteroploid subclones isolated from a heteroploid parent population, and among diploid subclones isolated from a diploid parent population. This variation is not related to differences in the growth ...

Abstract Genetic variation can be considered a driver of somatic evolution in glioblastoma (GBM); however, the tumor microenvironment (TME) is key contributor to selection process and evolutionary potential cancer cells. Although diverse ecosystems exist GBM which impact cell fitness characterize an aggressive pathophysiology, little known about how genetically distinct subpopulations cells int...

Plant tissue culture is an alternative method of commercial propagation and is being used widely for the commercial propagation of a large number of plant species, including many medicinal plants. Somatic embryogenesis is a process by which asexual or somatic cells are induced to form embryos in culture. Somatic embryogenesis is a multi-step regeneration process starting with formation of pro-e...

Induction of somatic embryogenesis in absence of exogenous auxin can clarify some basic concepts of somatic embryogenesis.  Explants from leaf and petiole of Cucumber (Cucumis sativus) were used in this experiment for somatic embryogenesis using three different modified B5 basal media; boron and 2,4-D but no IAA as control (I), boron and IAA but no 2,4-D (II) and no boron, 2,4-D and IAA (III). ...