Abstract Reverse phase dispersive liquid- liquid microextraction (RP- DLLME) coupled with spectrophotometry is introduced for the detection of total phenol in the muscle tissue of fishes, such as Sphyraena genie, Otolithes ruber, Rastrelliger kangurta, Lutjanus johnii and Barbus Subquin Cunciatus. Phenols in fish tissues were extracted by ultrasonic bath and derivatization with 4- aminoant...

The penicillin allergy is being increasingly recognized as a significant public health problem. Immunological responses to penicillin and other beta-lactam antibiotics are classified as immediate and non-immediate responses. This research aimed to develop an enzyme-linked immunosorbent assay (ELISA) for the detection of the reactive antibody value against penicillin in various species of animals...

The bioaccumulations of metals Cu, Cd, Ni, Cr, Co, Mn, Zn and Fe were measured in bivalves, Cerastoderma glucaum, and four species of fishes including Alburnus chalcoides, Liza aurata, Rutilus frisii and Sander lucioperca from various trophic levels of the Caspian food web. The concentrations of Cd, Cr, Co and Ni in most samples of fish were below the detection limits; while the concentrations ...

Background and Purpose: By using advanced detection/identification methods, the list of emerging uncommon opportunistic yeast infections is rapidly expanding worldwide. Our aim in the present study was sequence-based species delineation of previously unidentified yeasts obtained from a clinically yeast collection. Materials and Methods: A total of twenty three out of the 855 (5.7%) yeast isolat...

The present investigation was undertaken to describe the relationships among species of Avicenniaceae family which have both economic and medicinal activities, collected in Indian Sundarban by the help of RAPD and ISSR markers. In this study, three different species of Avicennia genus were collected. Frozen young leaves (-200C) were taken to isolate the genomic DNA using a slightly modified CTA...

Dactylogyrus spp. are monogenean worms found mostly as ectoparasites on the gills of several fish species, including carp and goldfish. These parasites are commonly detected by microscopic analysis of the gill lamellae, but this is time-consuming and technically difficult. In contrast to this conventional method, molecular techniques provide specific, sensitive and safe detection of parasites. ...

Objective During the last three decades sesquiterpenoid lactones have emerged as one of the largest groups of plant products with over 3000 naturally occurring substances known. The genus Artemisia characteristically contains this biologically active type of secondary metabolites. With respect to increasing interest in the cytotoxic, antibiotic, phototoxic and antineoplastic activities of sesq...

Ovine anaplasmosis is a tick-borne rickettsial disease, widespread in tropical and subtropical areas. In the present study, a PCR-RFLP method based on major surface protein 4 (MSP4) gene, was utilized for the detection of Anaplasma infection in 119 sheep blood samples collected from different parts of Ahvaz in the southwest of Iran. PCR identified Anaplasma infections in 87.4% (104/119) of the ...