Monoclonal anti-idiotypic antibodies generated against idiotypic immunoglobulin (Ig) of neoplastic B lymphocytes can be selected from growing hybridoma clones by their ability to recognize idiotypic but not normal IgM. This group of antibodies can be subdivided into those that bind to the target tumor cells in the presence of normal human serum (approximately 85% of the clones) and those in whi...

Most, if not all, malignancies have recurrent and disease-specific clonal chromosomal abnormalities, which play a pivotal role in tumor development. These comprise deletions or amplifications involving entire chromosomes or subchromosomal regions and, also, translocations. The latter represent the juxtaposition of fragments of DNA that are usually on different chromosomes. Most translocations a...

Conhecer e conservar as variedades de mandioca da Região do Vale Juruá, que tem a Identificação Geográfica (IG) farinha mandioca, montar uma coleção são necessários para haja manutenção recurso genético. O objetivo deste artigo foi prospectar diversidade genética detém IG Cruzeiro Sul visando à implantação germoplasma. Foram realizadas coletas 24 variedades. Para extração DNA, foram coletadas f...

The identification and tracking of antigen-specific immunoglobulin (Ig) sequences within total Ig repertoires is central to high-throughput sequencing (HTS) studies of infections or vaccinations. In this context, public Ig sequences shared by different individuals exposed to the same antigen could be valuable markers for tracing back infections, measuring vaccine immunogenicity, and perhaps ult...

This paper presents a comparison of traditional thermal and chemical reduction methods with more recent ionizing radiation via gamma rays electron beams (e-beams). For GO, all synthesis protocols were adapted to increase production scale are contribution this work. The typical Raman D-band the GO was prominent (ID/IG ratio increased sixfold). When comparing techniques, dramatic differences in e...

B cell-deficient (anti-mu-treated) mice have proven to be a valuable tool with which to examine the influence of Ig idiotypic determinants upon the development of the Ts repertoire. We have previously reported that ABA-specific Ts repertoires matured in normal and Ig-deficient environments differ from one another in their composition, and consequently, their functionally expressed Igh restricti...

We recently described the cloning of murine triggering receptor expressed by myeloid cells (TREM) 2, a single Ig domain DNAX adaptor protein 12-associated receptor expressed by cells of the myeloid lineage. In this study, we describe the identification of ligands for TREM-2 on both bacteria and mammalian cells. First, by using a TREM-2A/IgG1-Fc fusion protein, we demonstrate specific binding to...

The Down syndrome cell adhesion molecule, also known as Dscam, is a member of the immunoglobulin super family. Dscam plays an essential function in neuronal wiring and appears to be involved in innate immune reactions in insects. The deduced amino acid sequence of Dscam in the crustacean Pacifastacus leniusculus (PlDscam), encodes 9(Ig)-4(FNIII)-(Ig)-2(FNIII)-TM and it has variable regions in t...

BACKGROUND Post-transplant food allergy (LTFA) is increasingly observed after paediatric liver transplantation (LT). Although the immunopathology of LTFA remains unclear, immunoglobulin (Ig) E seems to be implicated. OBJECTIVE To study humoral and cellular immunity in paediatric LT patients in search for factors associated with LTFA, and compare with healthy controls (HC) and non-transplant f...

MHC class I-specific inhibitory receptors are expressed by a subset of memory-phenotype CD8(+) T cells. Similar to NK cells, MHC class I-specific inhibitory receptors might subserve on T cells an important negative control that participates to the prevention of autologous damage. We analyzed here human CD8(+) T cells that express the Ig-like MHC class I-specific inhibitory receptors: killer cel...