Y alphoid primers in combination with Alu and LINEs primers generated new DNA fragments in polymerase chain reactions (PCR) on DNA from a Y-only somatic cell hybrid but not from X-only, 3-only, or 21-only hybrids. X alphoid primers used in a similar manner generated new DNA fragments from the X-only hybrid, and 1 of the primers (X2) also generated new DNA fragments on 3-only and 21-only hybrids...

A two-step strategy, named exclusive PCR or E-PCR, has been developed to overcome the main limitation of PCR, which is the detection of already-known sequences only. This strategy allows the ability to detect and further clone and sequence genes for which no specific primers are available and in which a variable region exists between two conserved regions. This approach has been applied to Baci...

Background and purpose: Synthetic fertilizers damage the environment. Biofertilizers that consist of microorganisms emerge as an environmentally friendly alternative. improve plant growth by mobilizing soil nutrients, triggering hormone synthesis, or competing with pathogenic bacteria. However, biofertilizers often fail due to insufficient colonization roots.Materials methods: To explore dynami...

As a follow-up to our effort establish reliable thermodynamic data for amino acids, the heat capacity and phase behavior are reported N-acetyl glycine amide (CAS RN: 2620-63-5), N-acetyl-L-alanine 15962-47-7), N-acetyl-L-valine 37933-88-3), N-acetyl-L-isoleucine 56711-06-9), N-acetyl-L-leucine 28529-34-2). Prior measurement, thermogravimetric analysis X-ray powder diffraction were performed det...