Traditional spectrophotometry requires placing samples into cuvettes or capillaries. This is often impractical due to the limited sample volumes often used for protein analysis. The Thermo Scientific NanoDrop 2000c Spectrophotometer solves this issue with an innovative sample retention system that holds microvolume samples between two measurement surfaces using the surface tension properties of...

We are developing timeand energy-resolved near-IR/optical/UV photon detectors based on sharp superconducting-normal transition edges in thin films. We report observations of the Crab pulsar made during prototype testing at the McDonald 2.7m telescope with a fiber-coupled transition-edge sensor (TES) system. These data show substantial (δα ∼ 0.3), rapid variations in the spectral index through t...

We describe a simple, highly reproducible kinetic technique for precisely measuring temperature in spectrophotometric systems having reaction cells that are inaccessible to conventional temperature probes. The method is based on the temperature dependence of pseudo-first-order rate constants for the acid-catalyzed hydrolysis of N-o-tolyl-D-glucosylamine. Temperatures of reaction cuvette content...

There are a large number of assays in cellular and molecular biology that either produce or consume inorganic phosphate. Therefore, the ability to quantitatively measure these phosphorous compounds can allow the assessment of the associated enzymes. Here we describe the use of the mQuant Scanning microplate spectrophotometer to quantitate ATPase activity by measuring inorganic phosphate in solu...

A virtual chemical spectrophotometer for the simultaneous analysis of nickel (Ni) and cobalt (Co) was developed based on an artificial neural network (ANN). The developed ANN correlates the respective concentrations of Co and Ni given the absorbance profile of a Co-Ni mixture based on the BeerâĂŹs Law. The virtual chemical spectrometer was trained using a 3-layer jump connection neural network ...

An affordable and portable machine is required for the practical use of visible and near-infrared (Vis-NIR) spectroscopy. A portable fruit tester comprising a Vis-NIR spectrophotometer was modified for use in the transmittance mode and employed to quantify triglyceride levels in serum in combination with a chemometric analysis. Transmittance spectra collected in the 600- to 1100-nm region were ...

this paper investigates host condition factor, parasite interaction and metal accumulation in tilapia guineesis and host-parasite system in iddo area of lagos lagoon, nigeria. eighty specimens of t. guineensis from the lagoon were caught and dissected for intestinal helminth parasites. condition factors of all individuals were determined. median condition (k<2.62 and k>2.62) were used in groupi...