Objective: To develop a simple and cheap UV spectrophotometric method for the quantitative estimation of Indinavir sulphate (400mg) in capsules and validate as per ICH guidelines. Methods: The optimized method uses a diluent 100% potassium dihydrogen ortho phosphate buffer (pH 4.0) for the estimation of assay of Indinavir sulphate whose λmax is 259 nm. Results: The developed method resulted in ...

In the present work simple, precise, specific, accurate and cost effective UV spectrophotometric method has been developed for the determination of Mirabegron in pure and pharmaceutical formulation. The wavelength of the Mirabegron detection was 251 nm using methanol as a solvent and obeys Beer’s law in the concentration range of 0.2 – 1.2 μg /mL with a correlation coefficient of 0.9991. Relati...

This work presents the development of a methodology based on the formation of a charge transfer complex between quinalizarin and rosuvastatin, allowing for the spectrophotometric determination of rosuvastatin at 579 nm. The factors involved in the sensitivity of the technique were studied (nature and proportion of the solvent, reaction time, pH of aqueous phase and quinalizarin concentration). ...

Four simple, specific, accurate and precise spectrophotometric methods are developed and validated for simultaneous determination of cinnarizine (CIN) and dimenhydrinate (DIM) in a binary mixture with overlapping spectra, without preliminary separation. The first method is dual wavelength spectrophotometry (DW), the second is a ratio difference spectrophotometric one (RD) which measures the dif...

New selective and sensitive spectrophotometric and spectrofluorometric methods have been developed and validated for the determination of amantadine hydrochloride (AMD) in capsules and plasma. The methods were based on the condensation of AMD with 1,2-naphthoquinone-4-sulphonate (NQS) in an alkaline medium to form an orange-colored product. The spectrophotometric method involved the measurement...

Four new methods using titrimetry and spectrophotometry are described for the rapid determination of amoxycillin in bulk drug and dosage forms. In direct titrimetry (Method A), the drug is titrated with bromate–bromide mixture in acid medium using methyl orange indicator. Back titrimetry (Method B) entails adding a known excess amount of bromatebromide mixture to the drug solution in acid mediu...

We describe a liquid-chromatographic procedure for detection in urine of all thiazide drugs currently used clinically. Urine is treated initially with NaBH4 to convert any chlorothiazide present to hydrochlorothiazide. The urine is acidified with NaH2PO4 (1.0 mol/L, pH 5), and thiazides are extracted with ethyl acetate. Interfering substances are then removed in two washes with 0.1 mol/L Na2HPO...

The enzymatic acetylation of aromatic amines (1, Z), choline (3), glucosamine (4), and histamine (5) has been shown to require coenzyme A (CoA) ; recent studies (6) have demonstrated that the thio ester, acetyl CoA, can serve as the acetyl donor in the acetylation reaction. However, more detailed studies on the properties of the acetylating enzyme were made difficult by the absence of a suitabl...