tau.amegroups.com © Translational Andrology and Urology. All rights reserved. We read, with great interest, the commentary written by Wayland Hsaio on the “Clinical utility of sperm DNA fragmentation testing: practice recommendations based on clinical scenarios” (1). The author acknowledges sperm DNA fragmentation as an important addition to conventional methods for assessment of sperm quality ...

OBJECTIVES To examine the clinical value of six seminal plasma components in the evaluation of sperm quality and in the differential diagnosis of men with infertility. METHODS We analyzed 202 seminal plasmas for prostate-specific antigen, glucose, pepsinogen C, insulin-like growth factor binding protein-3, prostaglandin D synthase (PGDS), and BRCA1-like immunoreactive protein (BRCA1-LIP) usin...

It is my pleasure to write the foreword to this focused edition on sperm DNA fragmentation. This topic is one of the most exciting in male fertility diagnosis and treatment today and it has still so much untapped potential. After years of struggling with outdated diagnostic tools we have discovered DNA fragmentation; a sensitive and specific property of sperm. It is disappointing that there has...

BACKGROUND Sperm selection method is usually used to collect these cells for in vitro-assisted reproduction. Few studies reported the relationship of in vivo fertility and semen parameters after sperm selection; hence, the present study attempted to assess different semen parameters after post-thaw or sperm selection, using density gradient separation BoviPure®, to predict in vivo fertility. ...

The aim of the present study was to evaluate the effect of sperm reactive oxygen species (ROS) production and DNA changes on male fertility. For that purpose, six rams with significantly different pregnancy rates were used; these were classified as having high fertility, i.e. 59.4% average pregnancy rate, or low fertility, i.e. 23.1% average pregnancy rate. Sperm quality was assessed after a tw...

Background: Avian spermatozoa are characterized by high proportions of poly unsaturated fatty acid, associated with increased susceptibility to Reactive Oxygen Species and lipid peroxidation. Recent advances in poultry reproduction have focused on the potential of Reactive Oxygen Species as one of the prime mediators of infertility. Oxidative stress is the result of imbalance between the Reacti...

Purpose: To evaluate the drop-to-drop and pellet-to-pellet repeatability and stability of frozen sperm pellets. Methods: Ten pellets were thawed per batch (low and normal concentration) and evaluated by two investigators to establish a quality control chart. Then low and normal concentration pellets were thawed and evaluated daily for 10 days by both investigators. The values for both investiga...

Infertility is a problem affecting an increasing number of couples worldwide. Currently, marker tests for male factor infertility are complex, highly technical and relatively subjective. Up to 40% cases currently diagnosed as idiopathic therefore, there clear need further research into better ways diagnosing it. Changes in sperm telomere length have been associated with closely linked DNA damag...

Predicting the outcome of in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) is one main goal of the present research on assisted reproduction. To understand whether density gradient centrifugation (DGC), used to select sperm, can affect sperm DNA integrity and impact pregnancy rate (PR), we prospectively evaluated sperm DNA fragmentation (sDF) by TUNEL/PI, before and after DG...

Abnormal spermatozoa frequently display typical features of oxidative stress, i.e. excessive level of reactive oxygen species (ROS) and depleted antioxidant capacity. Moreover, it has been found that a high level of oxidatively damaged DNA is associated with abnormal spermatozoa and male infertility. Therefore, the aim of our study was the comparison of oxidative stress/DNA damage in semen and ...