Previous reports suggested that culture as 3D aggregates or as spheroids can increase the therapeutic potential of the adult stem/progenitor cells referred to as mesenchymal stem cells or multipotent mesenchymal stromal cells (MSCs). Here we used a hanging drop protocol to prepare human MSCs (hMSCs) as spheroids that maximally expressed TNFalpha stimulated gene/protein 6 (TSG-6), the antiinflam...

Recent progress on the structure and dynamics of bulges is reviewed. Those aspects that link galaxy bulges either to oblate spheroids akin to elliptical galaxies or to rapidly-rotating, flattened systems more nearly resembling the products of disk internal transformations, are highlighted. The analysis of surface brightness profiles derived from HST data is reviewed to show that unresolved nucl...

Patient-derived in vitro cultures of colorectal cancer (CRC) may help guide treatment strategies prior to patient treatment. However, most previous studies have been performed on a single biopsy per tumor. The purpose of this study was to analyze multiple spatially distinct biopsies from CRCs and see how well intratumor heterogeneity (ITH) was recapitulated in matching patient-derived spheroids...

BACKGROUND The aggressiveness of melanoma tumors is likely to rely on their well-recognized heterogeneity and plasticity. Melanoma comprises multi-subpopulations of cancer cells some of which may possess stem cell-like properties. Although useful, the sphere-formation assay to identify stem cell-like or tumor initiating cell subpopulations in melanoma has been challenged, and it is unclear if t...

Ability to grow under anchorage-independent conditions is one of the major hallmarks of transformed cells. Key to this is the capacity of cells to suppress anoikis, or programmed cell death induced by detachment from the extracellular matrix. To model this phenomenon in vitro, we plated Ewing tumor cells under anchorage-independent conditions by transferring them to dishes coated with agar to p...

The present study describes the effects of 2 folate antagonists, methotrexate (MTX) and the lipophilic antifolate trimetrexate (TMX) on 2 permanent human glioma cell lines (GaMg and D-54Mg) grown as monolayers and as multicellular tumor spheroids. In addition, the effects of drug exposure on tumor cell invasion was studied using a three-dimensional organ co-culture system. In monolayer cultures...

UNLABELLED It has been long recognized that the primary advantage of imaging the brain with a positron emission tomography using GSO scintillation detectors placed on a spheroid surface is the large solid angle of acceptance for annihilation radiation, which results in improved system sensitivity and image signal-to-noise ratio. In the present study, we investigated spheroid system geometry, de...

The effect of hyperthermia on the host-tumor interrelation ship was investigated using an in vitro-in vivo multiceli spheroid tumor model. The experiments were designed to attempt to discriminate between the relative importance of direct effects of heat on the tumor cells and of indirect delayed effects possibly related to modifications of immune responses to heated tumors or of damage to tumor...

As a suitable model to study the growth behavior and therapeutic response of drug-resistant and -sensitive cells in three-dimensional coculture we have established multicellular spheroids generated from both cisplatin-sensitive and -resistant cells of a murine fibrosarcoma cell line. A drug resistant clone was derived from the parent cisplatin-sensitive cells by intermittent drug exposure in vi...