UNLABELLED Deletions at the C-terminus of the proto-oncogene protein c-Src kinase are found in the viral oncogene protein v-Src as well as in some advanced human colon cancers. They are associated with increased kinase activity and cellular invasiveness. Here, we analyzed the mRNA expression signature of a constitutively active C-terminal mutant of c-Src, c-Src(mt), in comparison with its wild-...

In the context of non parametric multivariate regression model, we are interested in goodness-of-fit testing for single-index models. These models dimension reduction and therefore useful multidimensional nonparametric statistics because well-known phenomenon called curse dimensionality. Fan Li [5] have proposed first consistent test by using kernel estimation method a central limit theorem deg...

Dimensionality reduction (DR) methods have been commonly used as a principled way to understand the high-dimensional data such as facial images. In this paper, we propose a new supervised DR method called Optimized Projection for Sparse Representation based Classification (OP-SRC), which is based on the recent face recognition method, Sparse Representation based Classification (SRC). SRC seeks ...

Amplification of the HER-2/neu (ErbB2) gene is observed in approximately 30% of human breast cancers, correlating with a poor clinical prognosis. Src kinases are also involved in the etiology of breast cancer, and their activation was suggested to be necessary for Neu-induced oncogenesis. To address whether Src activity is essential for Neu-mediated tumorigenesis, we used a physiologic inhibito...

Physiological stimuli activate protein kinases for finite periods of time, which is critical for specific biological outcomes. Mimicking this transient biological activity of kinases is challenging due to the limitations of existing methods. Here, we report a strategy enabling transient kinase activation in living cells. Using two protein-engineering approaches, we achieve independent control o...

Both SRC-1 and TIF2 are members of the p160 steroid receptor coactivator family. Genetic analyses have shown that inactivation of TIF2, but not SRC-1, reduces postnatal survival, growth, and male reproductive function. Here, we demonstrate that, through analyses of SRC-1/TIF2 compound mutant mice, SRC-1 can partially compensate for the effects of a loss of TIF2 on mouse survival and growth, whe...

Tinsley, John H., Elena E. Ustinova, Wenjuan Xu, and Sarah Y. Yuan. Src-dependent, neutrophil-mediated vascular hyperpermeability and -catenin modification. Am J Physiol Cell Physiol 283: C1745–C1751, 2002. First published July 24, 2002; 10.1152/ajpcell.00230.2002.—The hyperpermeability response of microvessels in inflammation involves complex signaling reactions and structural modifications in...

We have shown that ouabain activates Src, resulting in subsequent tyrosine phosphorylation of multiple effectors. Here, we tested if the Na+/K+-ATPase and Src can form a functional signaling complex. In LLC-PK1 cells the Na+/K+-ATPase and Src colocalized in the plasma membrane. Fluorescence resonance energy transfer analysis indicated that both proteins were in close proximity, suggesting a dir...

Overexpression and activation of c-Src protein have been linked to the development of a wide variety of cancers. The molecular mechanism(s) of c-Src overexpression in cancer cells is not clear. We report here an internal ribosome entry site (IRES) in the c-Src mRNA that is constituted by both 5’ noncoding and coding regions. The inhibition of capdependent translation by m 7 GDP in cell-free tra...

Steroid receptor coactivators* SRC-1/NCOA1; SRC-2/NCOA2; (GRIP1, TIF2); SRC-3/NCOA3; (AIB1, ACTR, TRAM-1, RAC3) SRC-1 AR, COUP-TFI, CAR, ERα, ERβ, ERRg, FXRα, GR, HNF4α, PPARα, PPARg, PXR, PR, RORβ, RARα, RARβ, RXRα, RXRβ, SF-1, TRα, TRβ, EAR Coactivators for the NR superfamily and other transcription factors; roles in reproductive development and metabolism. Domains: PAS/ bHLH, acetyltransfera...