UNLABELLED The embryological, structural and functional unit of the dentine-pulp complex shares the odontoblast, located in the border of the dentine pulp, with basal nuclei and organelles. The odontoblast process emerges from its apical pole. It is formed by microtubules, microfilaments and vesicles covered by membranes penetrating the dentinal tubules, isolated from the inter-tubular matrix, ...

on the capacity of tissue elements to strain metachromatically. Surprising as it may seem, adequately fixed and dehydrated tissues withstand five minutes' immersion in concentrated sulphuric acid without charring or showing any severe disturbance of their structure. Subsequent staining with a metachromatic dye reveals that a greatly increased range of tissue elements shows a metachromatic affin...

We have modified the periodic acid-Schiff staining technique for glycoproteins for use with thin agarose-film electrophoresis membranes. With this procedure, carbohydrate-rich proteins can be detected with negligible background staining and insignificant staining of nonglycoproteins such as albumin and nonglycosylated Bence Jones proteins (free light chains). On the other hand, carbohydrate-ric...

Faecal samples from 291 dairy calves from 10 dairy herds in Kerman were examined to investigate theprevalence of Cryptosporidium infection. Cryptosporidium spp. oocysts were identified by using themodified Ziehl-Neelsen staining technique in 63 (21.65%) calves. Calves were grouped according to theirage as follows:

Guidelines recommend the roll-plate technique for short-term central venous catheter (CVC) tip cultures. However, the issue of whether the roll-plate technique is better than the sonication method for long-term CVCs remains unresolved. In addition, no data are available for predicting the value of direct Gram staining in anticipating catheter colonization or catheter-related bloodstream infecti...

Estrogen receptor (ER) was detected in frozen sections of 36 breast carcinomas using an antiestrophilin monoclonal antibody according to an immunocytochemical technique elaborated and made available by Abbott Laboratories in the form of a kit (ER-immunocytochemical assay monoclonal). Immunostaining was confined to the nuclei of the carcinoma cells. In all positive specimens, nuclei with differe...

An improved acid-fast staining technique for sputum examination for the primary diagnosis of tuberculosis is described. The technique was modified and simplified by the elimination of heating and by combining the stages of counterstaining: making the technique easier and safer, with less risk of phenol aerosols. The efficiency of this method was evaluated by comparison with two conventional met...

Image processing is the most effective method for enhancement and segmentation of tuberculosis bacilli in sputum smear samples. Improper straining can result poor screening results such as over-staining, under-staining, blurred images. The goal to find an image technique that will prepare feature extraction. There are still some shortcomings with existing when it implemented on Ziehl Neelsen In...