there is a growing concern about the application of molecular methods in epidemiological studies of infectious diseases. the objective of this study was to determine the genetic stability of methicillin resistance genes in staphylococcus aureus for the evaluation of resistance strain distribution. one hundred and fifteen s. aureus isolates from patients with staphylococcal infection were collec...

conclusions methicillin-resistant staphylococcus aureus is an increasingly common cause of nosocomial infections. our results are in agreement with those of other studies reporting that a few specialized clones are responsible for most cases of mrsa nasal carriage. in this study, mrsa strains isolated from different wards of hospital were closely related when analyzed by coagulase gene typing. ...

background and objective: vancomycin is frequently the antibiotic of choice for the treatment of infections caused by methicillin-resistant staphylococcus aureus (mrsa). for the last years, the incidence of vancomycin-intermediate s. aureus (visa) and vancomycin-resistant s. aureus (vrsa) has been increased in various parts of the world. the present study was carried out to determine the presen...

conclusions the present study reports on a high rate of multi-drug resistance, the predominance of the frequency of class 1 integron, and the emergence of spa type t790 among iranian staphylococcus aureus strains. the results revealed that the dissemination of multi-drug resistance among staphylococcus aureus isolates may be associated with the presence of integrons. therefore, continuous surve...

conclusions data reported here indicated that there is no specific genetic combination beyond the quantity of biofilm biomass in s. epidermidis. biofilm biomass seemed to be controlled by rnaiii expression level. further interest should be directed to biofilm dispersal since it seems that the key difference in biofilm biomass ability of s. epidermidis strains relates to factors regulating this ...

conclusion: the outcome of this study show that spa and coa typing are suitable meth­ods for mrsa isolates typing because it is easy to use and interpret them, and that these methods can be useful in infection source detection and its control especially in epidemic situations. result: pcr results showed seven different patterns for staff and five different patterns for patients based on spa gen...

Coagulase-negative staphylococci (CNS) are the predominant cause of bovine intra-mammary infections. They can lead to chronic infections and were reported to significantly increase milk somatic cell counts. The goal of our study was to determine the species distribution and antimicrobial susceptibility profiles of CNS in bovine mastitis milk samples in Switzerland. Between March 2011 and Februa...

We isolated from aerobic and anaerobic blood culture bottles from a febrile patient, a Helicobacter-like Gram negative, rod-shaped bacterium that MALDI-TOF MS failed to identify. Blood agar cultures incubated in a microaerobic atmosphere revealed a motile Gram negative rod, which was oxidase, catalase, nitrate reductase, esterase, and alkaline phosphatase positive. It grew at 42°C with no detec...

Reverse transcription of cellular RNA was used to obtain sequences from regions of 18S and 26/28S ribosomal RNA for eight species of ascaridoid nematodes. Phylogenetic relationships among these species were inferred from the aligned sequences by maximum-parsimony and maximum-likelihood methods. Seventy-nine of the 168 sites that varied were phylogenetically informative in parsimony analysis. Ph...

In this report, a new series of fluoroquinolone agents which was derived from levofloxacin was synthesized and evaluated against Gram-positive (Bacillus subtillis, Staphylococcus aureus, Staphylococcus epidermidis and Micrococcus luteus) and Gram-negative (Esherichio coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Serratia marcescens) organisms. The results showed that some of the synth...