Metazoan replication-dependent histone mRNAs end in a conserved stem-loop rather than in the poly(A) tail found on all other mRNAs. The 3' end of histone mRNA binds a single class of proteins, the stem-loop binding proteins (SLBP). In Xenopus, there are two SLBPs: xSLBP1, the homologue of the mammalian SLBP, which is required for processing of histone pre-mRNA, and xSLBP2, which is expressed on...

Stem cells have remarkable self-renewal ability and differentiation potency, which are critical for tissue repair and tissue homeostasis. Recently it has been found, in many systems (e.g. gut, neurons, and hematopoietic stem cells), that the self-renewal and differentiation balance is maintained when the stem cells divide asymmetrically. Drosophila male germline stem cells (GSCs), one of the be...

objective: this study presents a simple method for isolation, expansion and purification of neonatal mouse spermatogonial stem cells. methods: we used enzymatic digestion to isolate a cell suspension of spermatogonia and sertoli cells from neonatal 2-day-old mice. the cells were cultured in dmem/f12 that contained 10% serum for two weeks. sertoli and spermatogonia cell characteristics were conf...

Inconsistent with the view that epidermal stem cells reside randomly spread along the basal layer of the epidermal rete ridges, we found that epidermal cells expressing stem cell markers in nonglabrous skin exist in direct connection with the distal end of the arrector pili muscle. The epidermal cells that express stem cell markers consist of a subpopulation of basal keratinocytes located in a ...

BACKGROUND Diabetic nephropathy (DN) represents the leading cause of end-stage renal disease. Current therapeutic strategies for DN are very limited, and none of them can stop end-stage renal disease progression. Stem cell-based therapy showed encouraging outcomes in kidney disease, including experimental DN. SUMMARY Both podocytes and proximal tubular epithelial cells play key roles in the p...

It has been shown that a urea-splitting enzyme is present in normal gastric juice (1). Its range of activity was at least hetween pH 1.4 and 8.3, and its greatest action was found to take place at pH 7.8. Its activity was slow, and continued over a space of several days; the greatest part had taken place by the end of the first 24 hours. Owing to these characteristics it was suggested that the ...

We calculate the sub-gap density of states of a disordered single-channel normal metal connected to a superconductor at one end (NS junction) or at both ends (SNS junction). The probability distribution of the energy of a bound state (Andreev level) is broadened by disorder. In the SNS case the two-fold degeneracy of the Andreev levels is removed by disorder leading to a splitting in addition t...