Two facts must be taken into consideration when quantifying fibronectin in biological samples: the sensitivity of the assay method and the appropriate handling of samples. A three-antibody, non-competitive ELISA was used in the present study. This procedure offers a simple, inexpensive and very sensitive technique for evaluating fibronectin: reproducible standard curves in the range 5-100 micro...

the use of flow cytometry in the clinical laboratory has grown substantially in the past decade. flow cytometric analysis provides a rapid qualitative and quantitative description of multiple characteristics of individual cells. for example, it is possible to detect the cell size and granularity, aspects of dna and rna content and the presence of cell surface and nuclear markers which are used ...

The aim of this study is to develop a standardized LC-MS/MS method for accurate measurement of desmosine (DES) and isodesmosine (IDS) in all body fluids as biomarkers for in vivo degradation of matrix tissue elastin in man and animals. A reproducible three-step analytical procedure: (1) sample hydrolysis in 6N HCl, (2) SPE by a CF1 cartridge with addition of acetylated pyridinoline as internal ...

Detection and identification of human body fluids represent very important aspects of forensic biology, since in the case of numerous offences, a firm determination of the DNA source (e.g. semen, saliva, epithelium) rather than establishing the DNA profile alone is of great significance. The presently employed routine methods are insufficient: there are no specific tests confirming the presence...

A metabolic model for cadmium has been formulated in terms of a flow scheme for cadmium among eight body compartments. The mathematical description of the flow of cadmium between compartments consits of a number of differential equations, and the accumulation of cadmium may thus be calculated. Coeffcients for the flow of cadmium were estimated from empirical data both from animals and man. The ...