We examined the effectiveness of antisense RNA (as RNA) strategies for metabolic engineering of Clostridium acetobutylicum. Strain ATCC 824(pRD4) was developed to produce a 102-nucleotide asRNA with 87% complementarity to the butyrate kinase (BK) gene. Strain ATCC 824(pRD4) exhibited 85 to 90% lower BK and acetate kinase specific activities than the control strain. Strain ATCC 824(pRD4) also ex...

Mixtures of biological control agents can be superior to individual agents in suppressing plant disease, providing enhanced efficacy and reliability from field to field relative to single biocontrol strains. Nonetheless, the efficacy of combinations of Pseudomonas fluorescens A506, a commercial biological control agent for fire blight of pear, and Pantoea vagans strain C9-1 or Pantoea agglomera...

the main cause of structural damage in buildings subjected to seismic actions is lateral drift. in almost all reinforced concrete (rc) structures, whether designed with walls or frames, it is likely to be the code drift limits that control the design drift. the design drift limits and their contribution to damage may be represented indirectly through the material strain limits. the aim of this ...

THE article by Kurtz and Morris 1 in this issue of Hypertension raises important issues with regard to the use of genetically hypertensive rats. These issues are 1) the origins of various stocks, 2) the definition and maintenance of the integrity of various stocks of interest in hypertension research, and 3) the construction, appropriateness, and use of control strains for comparison with hyper...

THE article by Kurtz and Morris 1 in this issue of Hypertension raises important issues with regard to the use of genetically hypertensive rats. These issues are 1) the origins of various stocks, 2) the definition and maintenance of the integrity of various stocks of interest in hypertension research, and 3) the construction, appropriateness, and use of control strains for comparison with hyper...

THE article by Kurtz and Morris 1 in this issue of Hypertension raises important issues with regard to the use of genetically hypertensive rats. These issues are 1) the origins of various stocks, 2) the definition and maintenance of the integrity of various stocks of interest in hypertension research, and 3) the construction, appropriateness, and use of control strains for comparison with hyper...

Research Article Pamela S. Galluch Roanoke College [email protected] Varun Grover Clemson University [email protected] Jason Bennett Thatcher Clemson University [email protected] Contemporary information and communication technologies (ICTs) such as e-mail and instant messaging create frequent interruptions in the workday, which can potentially reduce business productivity and incr...

Patellofemoral pain (PFP) is thought to be related to patellar maltracking due to imbalances in the knee extensor. However, no study has evaluated the in vivo biomechanical properties of the quadriceps tendon in PFP syndrome. Our purpose was to compare the biomechanical properties of the quadriceps tendons in vivo and noninvasively in patients with PFP syndrome to those of control subjects. The...

introduction: physiological, anthropometrical and thermal perceptual are the most important factors affecting thermoregulation of men and women in workplaces. the purpose of this study was determining the validity of a questionnaire method for assessing women’s heat strain in workplaces. methods: this cross‑sectional study was carried out on 96 healthy women. data were continuously collected ov...

This prospective four-wave study examined (i) the causal direction of the longitudinal relations among job demands, job control, sleep quality and fatigue; and (ii) the effects of stability and change in demand-control history on the development of sleep quality and fatigue. Based on results of a four-wave complete panel study among 1163 Dutch employees, we found significant effects of job dema...