MATERIALS 5x TBE electrophoresis buffer Polyacrylamide gels are poured and run in 0.5x or 1x TBE at low voltage (1-8 V/cm) to prevent denaturation of small fragments of DNA by Joulic heating. Other electrophoresis buffers such as 1x TAE (please see Agarose Gel Electrophoresis) can be used, but they are not as good as TBE. The gel must be run more slowly in 1x TAE, which does not provide as much...

Polyacrylamide gels are typically formed by polymerization of the monomer acrylamide crosslinked to the co-monomer, N,N’-methylenebis-acrylamide, commonly called BIS. This process is a freeradical polymerization that requires an initiator, usually ammonium persulfate, and a catalyst like N,N,N’,N’-tetraethylmethylenediamine (TEMED). A distinct advantage of polyacrylamide gel systems is that by ...

INTRODUCTION Polymer blends are of great interest because they provide an economical route to design and produce novel materials that meet the challenging requirements for industrial applications. Recently, a variety of experimental and theoretical work has been conducted as a means for producing materials with improved properties compared to those of its constituents. Some of the findings sugg...

a simple and efficient procedure has been developed for the synthesis of 2,3-dihydroquinazolin-4(1h)-ones derivatives under solvent-free conditions. this method uses the condensation of isatoic anhydride, aldehydes, and amines in the presence of a catalytic amount sulfonated porous carbon (spc). one of the important advantages of the new method is that the spc could be recycled and reused.

Background. Open wound treatment requires a use of bandage material to prevent the development pathogenic microflora and provide necessary conditions for tissue regeneration.
 The aim study was compare effectiveness polyacrylamide (PAA) dextran-graft-polyacrylamide (D-PAA) hydrogels loaded with silver nanoparticles (AgNPs), antibiotics, photosensitizers bacterial infection open wounds.&#x0...

Aquivion membrane displays improved properties as compared to Nafion membrane, partly due shorter side chains. However, some improvements are still necessary for proton exchange fuel cell operate at low relative humidity. To overcome this drawback, the addition of clay nanoparticle into matrix can be considered. In study, different composite membranes have been prepared mixing short-side-chain ...

chemical flooding, especially alkaline/surfactant/polymer flooding, is of increasing interest due to the world increasing oil demand. this work shows the aspects of using alkaline/surfactant/polymer as an enhanced oil recovery method in the porous media having a high dead-end pore frequency with various dead-end pore parameters (such as opening, depth, aspect ratio, and orientation). using glas...

We herein describe a method of depositing hemoglobin (Hb) and sulfonated polyaniline (SPAN) on GC electrodes that facilitate interfacial protein electron transfer. Well-defined, reproducible, chemically reversible peaks of Hb and SPAN can be obtained in our experiments. We also observed enhanced peroxidase activity of Hb in SPAN films. These results clearly showed that SPAN worked as molecular ...