The development of profound leukocytosis in a patient with leukemic reticuloendotheliosis (LRE) enabled us to obtain purified LRE cells for the investigation of their structural and functional characteristics. The LRE cells of our patient bore surface immunoglobulin and had complement receptors but did not bear Fc receptors and did not form rosettes with sheep erythrocytes. By electron microsco...

Surface properties are often hypothesized to be important factors in the development of safer forms of nanomaterials (NMs). However, the results obtained from studying the cellular responses to NMs are often contradictory. Hence, the aim of this study was to investigate the relationship between the surface properties of silica nanoparticles and their cytotoxicity against a murine macrophage cel...

Abstract Today bone tissue engineering is one of the most used technologies for treat bones injure. Materials containing hydroxyapatite and graphene have received much attention recently. The aim this study was preparation biogenic bioceramics modified by graphene-like structures investigation effect on structure properties material. Biogenic were successfully prepared chemical vapor deposition...

Surface and cytoplasmic antigens in retinoblastoma were examined by antisera prepared against two tissue-cultured retinoblastoma cell lines. In addition, several other antisera, including anti-rhodopsin, anti-rod outer segment, anti-large-molecular-weight protein, anti-S, and anti-P antigen, were also utilized in a complement-dependent cytotoxicity assay in order to explore the tumor cell surfa...

Results GMS nVP auNPs with > 75% entrapment efficiency & < 300nm particle size were prepared and surface modified nanoparticles showed >85% binding to the ligand. In cytotoxicity study nanoformulations showed higher cell viability at all time points as compared to NVP. Cell uptake study revealed higher uptake of nanoparticles as compared to NVP only. In vitro anti HIV assay of surface modified ...

The complement system is an integral component of the humoral immune system, and describes a cascade of interacting proteins responsible for the opsonization and lysis of foreign pathogens, in addition to the recruitment of immune cells. However, complement activation is also implicated in the progression and complication of immune dysfunctions such as sepsis. Microparticle (MP) biomaterials ca...

The growing potential of quantum dots (QDs) in biomedical applications has provoked the urgent need to thoroughly address their interaction with biological systems. However, only limited studies have been performed to explore the effects of surface charge on the biological behaviors of QDs. In the present study, three commercially available QDs with different surface coatings were used to syste...

In our previous study, we demonstrated that 3β-hydroxysterol Δ24-reductase (DHCR24) was overexpressed in hepatitis C virus (HCV)-related hepatocellular carcinoma (HCC), and that its expression was induced by HCV. Using a monoclonal antibody against DHCR24 (2-152a MAb), we found that DHCR24 was specifically expressed on the surface of HCC cell lines. Based on these findings, we aimed to establis...

Recently, many nanomedical studies have been focused on magnetic nanoparticles (MNPs) because MNPs possess attractive properties for potential uses in imaging, drug delivery, and theranostics. MNPs must have optimized size as well as functionalized surface for such applications. However, careful cytotoxicity and genotoxicity assessments to ensure the biocompatibility and biosafety of MNPs are e...

Inhibitors of glycosylation and carbohydrate processing were used to probe the functional consequences of specific, differential alterations in glycosylation of monoclonal IgG secreted by hybridoma clones. Neither the absence of glycosylation nor the presence of atypical oligosaccharides significantly influenced binding of the monoclonal antibody to the cell surface antigen recognized. However,...