Manipulation of DNA fibers is very useful technique for gene analysis such as Fluorescent In Situ Hybridization (FISH). In conventional methods for manipulation of DNA fibers, FISH efficiency often decreases due to the tight adhesion between DNA fibers and substrates. In this paper, we propose a micro glass-phonorecord with concentric grooves for suspending DNA fibers with centrifugal force. As...

Bacterial protoplasts, released from confinement within the rigid wall of whole cells, imbibe water, swell, and burst unless their suspending medium contains high concentrations of solutes which have difficulty crossing the osmotic barrier in the protoplast surface membrane (C. Weibull, Exptl. Cell Res. 9:139, 1955; P. Mitchell and J. Moyle, J. Gen. Microbiol. 20:434, 1959). Hence, protoplast p...

The deliberately prepared one ssDNA/one micelle complex has an unstable toroidal DNA-bound region and stable upper and lower hemispheres, and thus can self-assemble along the plane of the unstable toroidal region into free-suspending films.

BiUSTA, F. F. (U.niversity of Illinois, UTrbana), AND Z. -JOHN ORDAL. Lack of influence of suspending media on heat activation of Bacillus subtilis spores and absence of deactivation. Appl. Microbiol. 12: 111-114. 1964.-Bacillus subtilis strain 5230 endosl)ores suslended in a variety of suspending media at a concentration of ca. 108 per ml were heated at 95 and 75 C. The effect of the heating a...

1. The concentrations of NADP and NADPH(2) in homogenates of rat liver (expressed as mug./g. wet wt. of tissue homogenized) were compared with values obtained from intact samples of liver taken from the same female rat. With 0.25m-sucrose alone as the suspending medium, or in combination with tris buffer or 0.01-0.1m-nicotinamide, considerable decreases in the sum of the NADP+NADPH(2) concentra...

Magnetic levitation, which uses a magnetic field to suspend objects in a fluid, is a powerful and versatile technology. We develop a compact magnetic levitation platform compatible with a smart-phone to separate micro-objects and estimate the density of the sample based on its levitation height. A 3D printed attachment is mechanically installed over the existing camera unit of a smart-phone. Mi...

We develop a computationally efficient cytoskeleton-based continuum erythrocyte algorithm. The cytoskeleton is modeled as a two-dimensional elastic solid with comparable shearing and area-dilatation resistance that follows a material law (Skalak, R., A. Tozeren, R. P. Zarda, and S. Chien. 1973. Strain energy function of red blood cell membranes. Biophys. J. 13:245-264). Our modeling enforces th...