The membrane potential of guinea-pig spermatozoa was investigated by using the fluorescent dye, diS-C3-(5). The potential did not vary when K replaced Na in the suspending medium, and in a physiological salt solution the resting potential was found to be about +13 mV.

Self-retaining barbed sutures, innovations for nonsurgical facial and neck rejuvenation, are currently available as short APTOS threads or long WOFFLES threads. The author uses APTOS threads for malar rounding, facial tightening and firming, and uses WOFFLES threads as a sling, suspending ptotic facial tissues to the firm, dense tissues of the temporal scalp.

3H-labeled dibenzyldimethylammonium ion, applied as an indicator for the potential differences across the plasma membrane, enters the Ehrlich ascites-tumor cell during 2 h to an apparent steady-state concentration about twice that of the suspending medium. In the presence of valinomycin, the distribution ratio reached was increased to about four, the cells meanwhile undergoing a substantial los...

A system has been described in which the penetration of Rickettsia tsutsugamushi (Karp strain) into tissue culture cells can be quantitated, and the factors affecting this process studied. The results indicated that rickettsial penetration in vitro depended largely on the viability of the organisms. Certain components of the fluid environment such as the divalent cations and protein were found ...

Thin gels (<I mm) can be loaded conveniently, rapidly, and quantitatively by suspending the sample to be analyzed in a drop of agarose gel and simply placing the solidified drop on top of the stacking gel. With this method there is no lower limit to the size of the sample to be loaded or to the thinness of the gel to be employed. Using the silver stain quantitation of between 1 and 1000 @ample ...

The persistence of A. butzleri CCUG 30484 on various surfaces under 32% and 64% relative humidity suspended in physiological saline or nutrient broth to simulate relatively clean or soiled conditions was studied using various isolation techniques. Our study revealed that A. butzleri CCUG 30484 cells were able to survive for a considerable period of time, even after the droplet of suspending med...

The use of sucrose solutions as suspending media for preparation of tissue homogenates and subsequent centrifugal fractionation has become a widely accepted procedure. Recently, it has been claimed that addition of colloids to the medium results in better preservation of the morphological and enzymic integrity of the isolated fractions (4, 8, 15). Such media also have valuable applications duri...

By simply crushing mineral grains, suspending the powder in a liquid, and dispersing the suspension on a suitable substrate, it is possible to collect adequate compositional and structural information to identify an unknown phase using a scanning electron microscope (SEM) equipped with an energydispersive X-ray spectrometer (EDS) and an electron backscatter diffraction system (EBSD). This techn...

Images which are captured in outdoor scenes often degrade in quality due to atmospheric conditions induced by suspending particles like mist, haze, fog, etc. which scatter and absorb light before it reaches to the camera. The weather-degraded images directly affect the efficiency of computerized surveillance and monitoring system. In this paper, we propose an efficient haze removal technique wh...