Several publications from this laboratory have dealt in a preliminary fashion with the preparation and properties of highly purified polynucleotide phosphorylase from Azotobacter vinelan&i (2-4). A more detailed account of this work is presented in thii paper. Although polynucleotide phosphorylase preparations can catalyze the synthesis of a variety of homopolymers (polyadenylic acid, polyuridy...

Live trachoma agent (TE55) injected into the anterior chamber of the rabbit eye produced the following agent-specific ocular lesions: corneal opacity, corneal neovascularization, and iritis. Three intravenous injections of 1 mg of a potent interferon (IF) inducer, polyriboinosinic acid-polyribocytidylic acid complex (poly I:C), markedly suppressed the ocular lesions produced by the trachoma age...

We report here the presence of two enzymatic activities associated with highly purified preparations of polynucleotide phosphorylase from Micrococcus luteus. The first, a nuclease activity, which is not separated from the phosphorylase on hydroxylapatite, may be due to substitution of H2O for phosphate in the phosphorolysis reaction. The second activity, a deoxyadenylate kinase, the bulk of whi...