The lamellar body count (LBC) represents an alternative method to the TDx-FLM II (Abbott Laboratories, Abbott Park, IL), which is planned to be discontinued, for assessing fetal lung maturity. Our objective was to validate the LBC on 3 hematology analyzers (Coulter LH 750 and Coulter Ac.T diff2, Beckman Coulter, Brea, CA; and Sysmex XE-2100, Sysmex, Mundelein, IL) to serve as a template for oth...

INTRODUCTION The Mean platelet volume and platelet counts are indicators of thrombotic potentials, and risk factors for microvascular complications in diabetics. This study aimed to establish variations in platelet counts and mean platelet volume in type 2 diabetic patients on treatment and non-diabetic controls. METHODS This was an unmatched case-control study involving 200 participants cons...

A screeningprocedure is described which can be used to separate the great majority of normal personsfrom those with a lack of the enzyme galactose transferase. The method is designed to be used on dried blood spot eluates which remain after a phenylalanine determination has been performed. The optimum conditions for this test are described, and data are presented from a preliminary study of mor...

Introduction m a n y a c c e p t e d chemical methods for blood alcohol involve the oxidation of alcohol after a separation step1’ 2 and the subsequent determination of the excess of oxidant. On the other hand, enzymatic procedures1-4 are based on the conversion of diphosphopyridine nucleotide (DPN) to its reduced form (DPNH), catalysed by alcohol dehydrogenase (ADH) in the presence of ethyl-al...

As manual methods for estimating the concentration of iron in serum are replaced by automated methods it is not always appreciated that the results may not be identical. Babson and Kleinman (1967), in their expei iments with 'serum protein solutions', found that the AutoAnalyzer procedure of Young and Hicks (1965) gave iron results which did not agree with a manual method (Peters, Giovanniello,...

We describe a continuous-flow, automated determination of total cholesterol in serum, which is based on enzymatic hydrolysis of cholesterol esters, oxidation of cholesterol by cholesterol oxidase, and colorimetric measurement of liberated perioxide with 4-aminoantipyrine, phenol, and peroxidase. Free cholesterol is determined with the same AutoAnalyzer II manifold and reagents, except that chol...