target. Whereas our method involves the additional cost associated with the need for dual-labeling of the probes, it has the advantage of being compatible with performance under standard PCR conditions, regardless of the SNP to be assayed. This characteristic makes it feasible to assay simultaneously, on the same 96or 384-well plate, a variety of SNPs. Interestingly, we noted that our factor V ...

Background: Taqman one-step real-time PCR (RT-PCR) has special importance due to its high sensitivity and specificity in the diagnosis of infectious diseases such as viral infections. In recent pandemic SARS-CoV-2, diagnostic kits based on this method are commonly used for molecular detection. One main systematic errors that misinterpret results is using inaccurate internal control RT-PCR kits....

Dear Sir, As part of association studies on cancer risk ERBB2 Ile655Val is a frequently analysed polymorphism. Recent publications have analysed the association of the common ERBB2 single nucleotide polymorphism (SNP) Ile655Val with breast cancer risk using TaqMan allelic discrimination (1,2). However, this genotyping method appears to be inappropriate regarding Ile655Val as the adjacent varian...

BACKGROUND Viral load measurements are commonly used to monitor HCV infection in patients with chronic diseases or determining the number of HCV-genomes in serum samples of patients after sustained virological response. However, in some patients, HCV viral load in serum samples is too low to be detected by PCR, especially after treatment. OBJECTIVES The aim of this study was to develop a high...

Background: The present investigation was carried out on poultry carcasses brought to the Department of Veterinary Pathology, LUVAS, Hisar from Herpesvirus turkeys (HVT) vaccinated chicken flocks and suspected for viral neoplastic conditions basis necropsy. Conventional molecular techniques confirmation Marek’s disease (MD) were studied. For differential diagnosis other oncogenic infections imm...