OBJECTIVE To examine molecular genetics and prenatal diagnosis of beta-thalassaemia. METHODS The study was conducted at the COMSATS Institute of Information Technology, Sahiwal, Pakistan, from October 2012 to October 2013, and comprised families having children affected by thalassaemia and autosomal recessive b-thalassemia. Blood samples of thalassaemic children and their parents were collect...

A new method of genetic analysis has been devised. The method, amplification refractory mutation system (ARMS), has been used to genotype the J3.11 MspI restriction fragment length polymorphism (RFLP) closely linked to cystic fibrosis (CF). The DNA sequence for both alleles of this dimorphism has been used to design ARMS primers. These allow genotyping of DNA isolated from blood, Guthrie cards,...

We combined the amplification refractory mutation system (ARMS) and fluorescence polarization (FP) to give a homogeneous genomic DNA genotype analysis method. Oligonucleotide probes labeled with the fluorescein dyes fluorescein isothiocyanate and 5-([4,6-dichlorotriazin-2-yl]amino)fluorescein and the rhodamine dye 6-carboxyrhodamine were included in amplification mixes and were annealed to PCR ...

The purpose of this study was to identify Follicle Stimulating Hormone (FSH) gene mutation West Sumatera Pitalah ducks. This used blood samples from 50 ducks (5 males and 45 females). Extraction analized by Kit iNtRON Biotechnology amplification DNA extraction the primer with fragmen target 318 bp. Sequencing 1st Base Singapore product Dnastar. Based on result is ducks.
 Key Words: FSH, Ge...

Background & Aims:Thromboembolism is an acute cardiovascular disease that ranges from clinically unimportant to massive embolism. Both acquired and hereditary risk factors contribute to the disease.
We aimed to determine the prevalence of two hereditary predisposing factor of the disease, prothrombin G20210A and factor V Leiden (G1691A) polymorphisms, in Kerman population.<br /...

Numerous avian species are sexually monomorphic, especially nestlings, posing difficulties in determining their sex by morphological traits alone. Despite the difficulties, sex identification of birds is an essential part of ex situ conservation breeding programs for endangered species, and the sex of individuals is an integral component of information required for research concerning ecology, ...