We describe an efficient SCHEMA recombination-based approach for screening homologous enzymes to identify stabilizing amino acid sequence blocks. This approach has been used to generate active, thermostable cellobiohydrolase class I (CBH I) enzymes from the 390 625 possible chimeras that can be made by swapping eight blocks from five fungal homologs. Constructing and characterizing the parent e...

With the aim of identifying novel thermostable esterases, comprehensive sequence databases and cloned fosmid libraries of metagenomes derived from an offshore oil reservoir on the Norwegian Continental Shelf were screened for enzyme candidates using both sequence-and function-based screening. From several candidates identified in both approaches, one enzyme discovered by the functional approach...

Abstract Pectinases, like other industrial enzymes are usually expensive. The use of pineapple peel pectin as substrate is triggered by the large tones waste generated in Nigeria. Oil extraction mechanical/chemical means have associated disadvantages. This research aimed at employing locally produced pectinase for coconut-oil and to compare yield with commercial pectinase. Fifty grammes dried p...

The aim of this study was the use and revalorization two persimmon by-products A B generated in juice production process. by-product resulting from a pectinase enzymatic treatment peels pulp to optimize extraction especially suitable for recovery valuable bioactive carotenoids. solvents solvent combinations used were: ethanol, acetone, ethanol/acetone (50:50 v/v) ethanol/acetone/hexane (25:25:5...

A total of 627 clinical isolates of Micrococcaceae were characterized accoridng to their ability to coagulate rabbit and pig plasma, produce thermostable nuclease, and anaerobically ferment glucose and mannitol. By using these characteristics, 416 of the isolates were classified as Staphylococcus aureus and 211 as non-S. aureus. All 416 strains produced a 3 to 4+ clot formation in heparinized p...

Carbonic anhydrases are ubiquitous metallo-enzymes which catalyze the reversible hydration of carbon dioxide in bicarbonate ions and protons. Recent years have seen an increasing interest in the utilization of these enzymes in CO2 capture and storage processes. However, since this use is greatly limited by the harsh conditions required in these processes, the employment of thermostable enzymes,...

Terpenoids include structurally diverse antibiotics, flavorings, and fragrances. Engineering terpene synthases for control over the synthesis of such compounds represents a long sought goal. We report computational design, selections, and assays of a thermostable mutant of tobacco 5-epi-aristolochene synthase (TEAS) for the catalysis of carbocation cyclization reactions at elevated temperatures...