نتایج جستجو برای: throughput nucleotide sequencing

تعداد نتایج: 307622  

Journal: :Proceedings of the National Academy of Sciences 2010

2013
Nicola Segata Daniela Boernigen Timothy L Tickle Xochitl C Morgan Wendy S Garrett Curtis Huttenhower

Complex microbial communities are an integral part of the Earth's ecosystem and of our bodies in health and disease. In the last two decades, culture-independent approaches have provided new insights into their structure and function, with the exponentially decreasing cost of high-throughput sequencing resulting in broadly available tools for microbial surveys. However, the field remains far fr...

2008
Gerardo Turcatti Anthony Romieu Milan Fedurco Ana-Paula Tairi

Fluorescent 2'-deoxynucleotides containing a protecting group at the 3'-O-position are reversible terminators enabling array-based DNA sequencing by synthesis (SBS) approaches. Herein, we describe the synthesis of a new family of 3'-OH unprotected cleavable fluorescent 2'-deoxynucleotides and their evaluation as reversible terminators for high-throughput DNA SBS strategies. In this first versio...

2013
Liang Sun Haitao Luo Dechao Bu Guoguang Zhao Kuntao Yu Changhai Zhang Yuanning Liu Runsheng Chen Yi Zhao

It is a challenge to classify protein-coding or non-coding transcripts, especially those re-constructed from high-throughput sequencing data of poorly annotated species. This study developed and evaluated a powerful signature tool, Coding-Non-Coding Index (CNCI), by profiling adjoining nucleotide triplets to effectively distinguish protein-coding and non-coding sequences independent of known an...

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