Plant viruses have a significant impact on agronomic losses worldwide. A new strategy for engineering virus-resistant plants by transgenic expression of a dominant interfering peptide is presented here. This peptide of 29 aa strongly interacts with the nucleocapsid proteins (N) of different tospoviruses. Transgenic Nicotiana benthamiana lines expressing the peptide fused to a carrier protein we...

This study was initiated to determine the incidence and severity of fungal diseases tomatoes document farmers’ knowledge these their control measures in Offinso North, Techiman North Asante Akim Districts within forest forest-savannah agro-ecological zones Ghana. A survey conducted three selected communities each district tomato also practices adopted on farms using a well-structured questionna...

Few commercial genotypes of tomato are resistant to Fusarium wilt (Fusarium oxysporum f. sp. lycopersici [FOL]), especially race 3. We developed five intraspecific hybrids Solanum lycopersicum (FOX1 FOX5) and assessed them for agronomic traits resistance FOL races 1, 2, 3, a mixture these races. FOX1 FOX4 were all the but did not have desired traits. Next, as rootstocks cherry Sweet Heaven. The...

Verticillium wilt caused by soilborne fungus Verticillium dahliae could significantly reduce cotton yield. Here, we cloned a tomato Ve homologous gene, Gbve1, from an island cotton cultivar that is resistant to Verticillium wilt. We found that the Gbve1 gene was induced by V. dahliae and by phytohormones salicylic acid, jasmonic acid, and ethylene, but not by abscisic acid. The induction of Gbv...

Abstract Endophytic bacteria serve key roles in the maintenance of plant health and growth. Few studies to date, however, have explored antagonistic growth-promoting (PGP) properties Prunus cerasifera endophytes. To that end, we isolated endophytic from P. tissue samples used a dual culture plate assay screen these microbes for activity against Verticillium dahliae, Botryosphaeria dothidea, Fus...

fusarium wilt caused by fusarium oxysporum f.sp. lycopersici is one of the major obstacles to the production of tomato which causes huge losses in tomato products worldwide. in order to increase the tolerance to this disease, a triple structure containing pr1, chitinase and glucanase genes controlled by 35s promoter was transferred to tomato. eight days after planting on pre-culture medium, exp...

Abstract Plant intracellular nucleotide-binding domain leucine-rich repeat (NLR) receptors play crucial roles in immune responses against pathogens. How diverse NLRs recognize different pathogen effectors remains a significant question. A recent study published Nature uncovered how pepper NLR Tsw detects phytohormone receptors’ interference caused by tomato spotted wilt virus (TSWV) effector, t...

Ralstonia solanacearum is a causative agent of bacterial wilt in many important crops throughout the world. How to control bacterial wilt caused by R. solanacearum is a major problem in agriculture. In this study, we aim to isolate the biocontrol agents that have high efficacy in the control of bacterial wilt. Three new bacterial strains with high antimicrobial activity against R. solanacearum ...

The characterization of microbial communities that promote or suppress soil-borne pathogens is important for controlling plant diseases. We compared prokaryotic communities in soil with or without the signs of tomato bacterial wilt caused by Ralstonia solanacearum. Soil samples were collected from a greenhouse at two different depths because this pathogen is present in deep soil. We used sample...