We introduce two new variance estimation procedures by using non-overlapping and overlapping blocks, respectively. The non-overlapping block (NOB) estimator can be viewed as the limit of the thinned block bootstrap (TBB) estimator recently proposed in Guan and Loh (2007), by letting the number of thinned processes and bootstrap samples therein both increase to infinity. Compared to the latter, ...

the interaction between isatin-β-thiosemicarbazone (ibt) and calf thymus dna (ct-dna) was investigated in physiological buffer (ph 7.4) using neutral red (nr) dye as a spectral probe by uv–vis absorption and fluorescence spectroscopy, as well as viscosity measurements. the ibt is stabilized by intercalation in the dna ( k [ibt –dna] =1.03×105 m−1), and displaces the nr dye from the nr–dna comple...

Elevated levels of protein kinase CK2 were found in tumour cells compared to normal cells. Thus, inhibition of CK2 kinase activity seems to be an attractive method to stop growth of cancer cells. Two drugs, namely tetrabromobenzotriazole, TBB, and 2-dimethylamino-4,5,6,7-tetrabromo-1H-benzimidazole, DMAT were found to specifically inhibit CK2 and to induce apoptosis in tumour cells. The aim of ...

Incubation of Jurkat cells with 4,5,6,7-tetrabromobenzotriazole (TBB), a specific inhibitor of protein kinase CK2, induces dose-and time-dependent apoptosis as judged by several criteria. TBB-promoted apoptosis is preceded by inhibition of Ser/Thr phosphorylation of haematopoietic lineage cell-specific protein 1 (HS1) and is accompanied by caspase-dependent fragmentation of the same protein. Bo...

Protein kinase CK2 (formerly termed "casein kinase II") is a ubiquitously in mammalian cells distributed Ser/Thr kinase, with global role in cell regulation. Although, the involvement of CK2 in cell signalling is vast-investigated, virtually nothing is known about its contribution to signal control of keratinocytes differentiation. Here we show that, in autocrine differentiating keratinocytes, ...

For the recent CMOS feature sizes power dissipation becomes an overriding concerns for VLSI circuit design. We propose a novel approach named tri-state elastic buffer design which reduces the total power, area & delay of elastic buffer. The paper presents a design and implementation of tristate buffer mechanism in 120 nm technology. This design offers also the advantage of third state (High Imp...

Cisbio offers a comprehensive line of HTRF phosphoand total protein assays to investigate cell signaling pathways. These versatile, homogeneous assays are suitable for a variety of sample types, including primary cell lines and tumor xenografts. Each HTRF assay includes a common and essential step that relies on the ability of the lysis buffer to release the protein of interest. This protein, e...

The interaction between isatin-β-thiosemicarbazone (IBT) and calf thymus DNA (CT-DNA) was investigated in physiological buffer (pH 7.4) using Neutral Red (NR) dye as a spectral probe by UV–Vis absorption and fluorescence spectroscopy, as well as viscosity measurements. The IBT is stabilized by intercalation in the DNA ( K [IBT –DNA] =1.03×105 M−1), and displaces the NR dye from the NR–DNA comple...

European "Technical Recommendations" have proposed, in addition to the use of substance concentrations, a pH (≤ 2 or ≥ 11.5) and an acid / base buffering capacity classify waste into according their hazardous properties HP 4 'Irritant' 8 'Corrosive'. Buffer refers 2018 UK classification guide referring 'corrosive' level method proposed 1988 for substances preparations but not retained EU regula...

M a t e r i a l a n d M e t h o d s T he subjects examined comprised 90 Bushmen, 55 males and 35 females, from the Lone Tree and Kang districts of Bot­ swana. These districts form part of the Southern K alahari desert. S t im u la te d saliva sp ec im e n s were obtained from the subjects by their chew­ ing a cube of paraffin wax for three minutes and expectorating into a glass beaker when nece...