A case of transcobalamin II deficiency with several unique features is described. The clinical presentation was typical. except for a slightly delayed age at presentation and the occurrence of apparent neurologic dysfunction from the beginning. The unusual biochemical feature was a low serum cobalamin level (97 pg/mI). Several cobalaminbinding protein abnormalities coexisted and antedated cobal...

Delta-toxin produced by Clostridium perfringens types B and C is a β-pore-forming cytotoxin. Here, using site-directed mutagenesis, we identified the amino acid residues that contribute to delta-toxin oligomerization binding. We replaced Lys-43 Ser-109 located in β-sandwich domain Arg-200 rim domain. Substitution of alanine for caused reductions both cytotoxicity oligomerization. However, excha...

Two experiments were performed to screen bacilli isolated from quails for their aflatoxin removal potential and to assess the efficiency of their amelioration of experimental aflatoxicosis. Nonhemolytic bacilli were selected for in vitro aflatoxin B1 (AFB1) removal and conventional probiotic tests. The isolate with the highest scores was selected for assessment in field experiments and was iden...

A patient presented at the age of 77 yr with a low serum cobalamin level. Subsequent study showed that he had persistently very low R binder (TC I) cobalamin-binding capacity in serum (< 5 ng/liter versus 213 ± 171 ng/liter in normal controls). and that almost all of his endogenous serum cobalamin was carried by TC II instead of TC I. His saliva also demonstrated virtually undetectable R binder...

We investigate numerically on the lattice the interplay of universality classes of the threedimensional Yukawa model with U(1) chiral symmetry, using the Binder method of finite size scaling. At zero Yukawa coupling the scaling related to the magnetic Wilson–Fisher fixed point is confirmed. At sufficiently strong Yukawa coupling the dominance of the chiral fixed point associated with the 3D Gro...

This paper presents a simulation model of a binder of asymmetric digital subscriber lines (ADSL). The model is developed in the Simulink environment and enables the investigation of multicarrier communication aspects in the complex system of a loop binder interconnecting the customer premises with the nearby central office. Key features of the model architecture are the update of the model bloc...

background : clostridium perfringens type c strains cause severe necrotizing enteritis in sheep, calves, goats, pigs and humans. beta-toxin is introduced to be the essential virulence factor of this microorganism. in the present study, a new method was established for the purification of beta toxin from culture supernatant fluid of c. perfringens type c vaccinal strain. methods : the four steps...

objective: verotoxin is a member of shiga toxin family. this family contains ab protein toxins with an enzymatic (a) and a binding (b) compartment. cells that have receptor (gb3) are sensitive to cytotoxic effects of toxin. it has been shown that various tumor cells have gb3 receptor and are selectively sensitive to apoptotic effect of verotoxin. studies on tumor cell lines and laboratory anima...