Objectives: The objective of this study was to analyze the fatty acid content, antioxidant, and antibacterial activities of hydro-methanolic extract of Adonis wolgensis L. (A. wolgensis L.) growing wild in north of Iran. Materials and Methods: Oils of A. wolgensis L. was obtained by means of Soxhlet apparatus from leaves and stems. Methyl esters were derived from the oily mixtures by trans-este...

Conjugated linoleic acid (CLA) isomers in oils are currently measured as fatty acid methyl esters by a gas chromatography-flame ionization detector (GC-FID) technique, which requires approximately 2 h to complete the analysis. Hence, we aim to develop a method to rapidly determine CLA isomers in CLA-rich soy oil. Soy oil with 0.38-25.11% total CLA was obtained by photo-isomerization of 96 soy o...

There are no reported pathways of docosahexanoic (DHA) biohydrogenation; however, DHA is metabolized by ruminal microorganisms. In this study a 0.5mg pulse dose of uniformly 13C labeled DHA in ethanol was injected at 0h as a metabolic tracer. This study did not examine every fatty acid metabolite, but rather identified several saturated and trans-18:1 fatty acid metabolites and set a foundation...

According to various studies, fats of animal and vegetable origins satisfy 22% to 42% of the daily energy demands of human beings (Srinivasan et al., 2006; Wagner et al., 2008; Willet, 2006). Some fats, and especially those that are hydrogenated, contain trans fatty acids (TFAs), i.e. unsaturated fatty acids with at least one double bond in a trans configuration (Craig-Schmidt, 2006). This tran...

1. A number of rumen bacteria isolated because of their ability to deacylate phosphatidyl choline, were found, in addition, to hydrogenate polyunsaturated fatty acids. 2. The most active lipolytic organisms had an unusual pattern of hydrogenation of dietary fatty acids in that alpha-linolenic acid was hydrogenated only as far as trans-11, cis-15-octadecadienoic acid.

Conjugated linoleic acid (CLA) has been shown to reduce body fat mass in various experimental animals. It is valuable to identify its influence on enzymes involved in energy expenditure, apoptosis, fatty acid oxidation and lipolysis. We investigated isomer-specific effects of high dose, long treatment of CLA (75.4 μmol/L, 8 days) on protein and gene expression of these enzymes in cultured 3T3-L...

The study investigated the quantitative and qualitative [chemical] characteristics of fatty acids, carotenoids and tocopherol in some fast foods prepared from a Nigerian eatery. The fatty acid profile was determined by gas chromatography, carotenoids by open column chromatography and visible spectrophotometry, and tocopherol by colorimetric method. The free fatty acid, acid and peroxide values ...

Effects of butter and 2 types of margarine on blood lipid and lipoprotein concentrations were compared in a controlled diet study with 23 men and 23 women. Table spreads, added to a common basal diet, provided 8.3% of energy as fat. Diets averaged 34.6% of energy as fat and 15.5% as protein. Each diet was fed for 5 wk in a 3 x 3 Latin-square design. One margarine (TFA-M) approximated the averag...

A total of 113 samples of various types of palm and palm kernel oil products, their fractions, palm-based and non-palm-based cooking oils obtained from local manufacturers and the retail market were analysed for their trans-fatty acid compositions and contents by capillary gas chromatography. Trans-fatty acids were generally absent in crude palm and palm kernel oils. However, they were present ...