Bovine cardiac troponin-I (TN-I) and troponin-T (TN-T) have been examined in solution using ultracentrifugation, gel filtration, and viscosity. A new method of purifying TN-T, employing hydroxylapatite chromatography in 6 M urea, is reported. Cardiac TN-T (Mr = 36,000) undergoes a reversible, concentration-dependent association in nondenaturing buffers, probably to a tetramer. The Stokes radius...

Troponin, an important regulatory protein of the thin filament (actin) of striated muscle, is a complex of 3 subunits: C, T, and I. Troponin T and I isoforms from the heart are structurally different from the corresponding forms found in skeletal muscle. Because of this distribution, the measurement of cardiac troponin T and I isoforms is superior to other serum biomarkers of cardiac disease su...

1. Cooper LT, Jr. Myocarditis. N Engl J Med 2009;360:1526-38. 2. Kadota S, Takeuchi Y, Izuhara M, et al. The importance of serial cardiac troponin measurement for evaluating the response to immunosuppressive therapy for myocarditis. J Cardiol 2008;52:154-8. 3. Giannoni A, Giovannini S, Clerico A. Measurement of circulating concentrations of cardiac troponin I and T in healthy subjects: a tool f...

The essential role of cardiac troponin in the diagnosis of acute myocardial infarction has led to the development of high-sensitivity assays, which are able to detect very small amounts of myocardial necrosis. The high-sensitivity cardiac troponin T assay, however, is not entirely specific for myocardial injury. This case report describes a 48-year-old woman, who, two years after cardiac transp...

441 Abstract: Cardiac troponin T (TnT) is a regulatory contractile protein not normally found in blood. Minor elevations of TnT in patients with unstable angina have been associated with an increased risk of subsequent death or Acute Myocardial Infarction (AMI). We used a neewly developed enzyme immunoassay for TnT to determine whether its presence in the serum of patients with unstable angina ...

BACKGROUND The prognostic value of biochemical markers in relation to time since onset of chest pain was evaluated in an emergency room with a chest pain unit. METHODS In a single-center, prospective study we included 321 consecutive patients admitted to the emergency room with suspected unstable angina IIIB and an evolution of less than 12 hours. Blood samples were collected for CPK, CPK MB ...

Effects of phosphorylation of bovine cardiac troponin T (TnT) by protein kinase C on the Ca(2+)-stimulated MgATPase activity of reconstituted actomyosin complex and the binding of TnT to tropomyosin(Tm)-F-actin were investigated. The Ca(2+)-stimulated MgATPase of actomyosin containing phosphorylated TnT (1.8 mol of P/mol), compared with that containing unphosphorylated TnT, was decreased by up ...