The aim of this study was to reevaluate the ecology of an area in the Atlantic Forest, southeast Brazil, where Chagas disease (CD) has been found to occur. In a previous study, immediately after the occurrence of a CD case, we did not observe any sylvatic small mammals or dogs with Trypanosoma cruzi cruzi infections, but Triatoma vitticeps presented high T. c. cruzi infection rates. In this stu...

Trypanosomatidae is a group of protozoa belonging to Trypanosomatida order, where the genres of medical importance Leishmania and Trypanosoma are inserted. These genres include parasites Leishmania spp., Trypanosoma cruzi and Trypanosoma brucei, etiologic agents of leishmaniasis, Chagas disease and sleeping sickness, respectively classified as Neglected Tropical Diseases (NTDs) by the World Hea...

In Venezuela, two non-tsetse transmitted trypanosomes, Trypanosoma evansi and Trypanosoma vivax, are the major etiological agents of animal trypanosomosis. Rodents can be experimentally infected with T. evansi in order to obtain enough parasites to prepare antigens for serological tests. On the contrary, the production of T. vivax antigens is a limiting factor in most laboratories. Since T. eva...

Serological relationships between new isolates of Trypanosoma cruzi from a single locality have been examined by direct agglutination and immunodiffusion, and compared with established strains. The strains were divided into three groups according to the absorption properties of their precipitinogens. The three groups were defined by the same characteristics as those in which strains of wide-ran...

The probability of tsetse, Glossina pallidipes, acquiring a trypanosome infection from a single blood meal was estimated in five localities on the Kenya coast which were selected for differences in habitat and host availability. The probability that one blood meal contained infective Trypanosoma congolense was 0.0077 in rural areas with domestic animals, 0.0019 in extensive areas of natural hab...

Changes in pulsatile secretion of LH and testosterone and responses to exogenous GnRH were assessed at different stages of Trypanosoma congolense infection in rams. Jugular blood samples were collected every 15 min for 6 h followed by immediate injection of GnRH (20 micrograms i.v.) and further sample collection after 10, 20, 40, 60, 80, 100 and 120 min. This sampling and injection regimen was ...

BACKGROUND Trypanosoma brucei (T b) gambiense is targeted to reach elimination as a public health problem by 2020 and full elimination by 2030. To achieve these goals, stakeholders need to consider strategies to accelerate elimination. Hence, we aimed to model several options related to current and emerging methods for case detection, treatment, and vector control across settings to assess cost...

The aim of this study was to provide information on trypanosome species infecting trypanotolerant cattle from southern Gabon. The study was conducted on 224 trypanotolerant cattle from three regions located in southern Gabon, using ITS1 primer-based PCR. Seventy-two (32%) N'dama cattle were found polymerase chain reaction (PCR) positive with trypanosomes. The overall prevalence of trypanosomosi...

In order to understand the key parameters influencing drug susceptibility, different Trypanosoma cruzi assay protocols were evaluated using a comparative assay design. The assays compared in this study were an image-based intracellular T. cruzi assay quantified through an image-mining algorithm and an intracellular assay utilizing a β-galactosidase-expressing T. cruzi strain. Thirty-one referen...

Peptides derived from the surface glycoprotein gp82 of Trypanosoma cruzi, previously implicated in the parasite's invasion of host cells, were expressed as fusions to the protein LamB of Escherichia coli in a region known to be exposed on the cell surface. Bacteria expressing these proteins adhered to HeLa cells in a manner that mimics the pattern of parasite invasion of mammalian cells. Purifi...