A new method using high-performance liquid chromatography coupled with ultra violet detection (HPLC-UV) was developed and validated for the simultaneous quantification of atazanavir, dolutegravir, darunavir, efavirenz, etravirine lopinavir, raltegravir, rilpivirine and tipranavir in human plasma. For the first time we reported here the development and validation of an HPLC-UV assay to quantify ...

Membrane organelles often have complicated shapes and differ in their volume, surface area and membrane curvature. The ratio between the surface area of the cytosolic and luminal leaflets (trans-membrane area asymmetry (TAA)) determines the membrane curvature within different sites of the organelle. Thus, the shape of the organelle could be critically dependent on TAA. Here, using mathematical ...

Combining data from multiple analytical platforms is essential for comprehensive study of the molecular phenotype (metabotype) of a given biological sample. The metabolite profiles generated are intrinsically dependent on the analytical platforms, each requiring optimization of instrumental parameters, separation conditions, and sample extraction to deliver maximal biological information. An in...

Cymbidium kanran, an orchid exclusively distributed in Northeast Asia, has been highly valued as a decorative plant and traditional herbal medicine. Here, C. kanran extracts were prepared in 70% aqueous methanol using ultrasound-assisted extraction (UAE) and subjected to liquid chromatography-photodiode array detection and ultra-high performance liquid chromatography-quadrupole-time-of-flight-m...

We have developed and validated a novel, sensitive, selective and reproducible reversed-phase high-performance liquid chromatography method coupled with electrospray ionization mass spectrometry (HPLC-ESI-MS/MS) for the simultaneous quantitation of ceftriaxone (CEF), metronidazole (MET) and hydroxymetronidazole (MET-OH) from only 50 µL of human plasma, and unbound CEF from 25 µL plasma ultra-fi...

We have developed and validated a novel, sensitive, selective and reproducible reversed-phase high-performance liquid chromatography method coupled with electrospray ionization mass spectrometry (HPLC–ESI-MS/MS) for the simultaneous quantitation of ceftriaxone (CEF), metronidazole (MET) and hydroxymetronidazole (MET-OH) from only 50 μL of human plasma, and unbound CEF from 25 μL plasma ultra-fi...

We have developed and validated a novel, sensitive, selective and reproducible reversed-phase high-performance liquid chromatography method coupled with electrospray ionization mass spectrometry (HPLC–ESI-MS/MS) for the simultaneous quantitation of ceftriaxone (CEF), metronidazole (MET) and hydroxymetronidazole (MET-OH) from only 50 μL of human plasma, and unbound CEF from 25 μL plasma ultra-fi...

In this paper, the extraction of polyphenols from amaranth seed using a Box–Benhken design four factors—ultra-turrax speed, solid-to-liquid ratio (RSL), methanol concentration and time—were studied. There were two responses studied for model: total phenolic content (TPC) flavonoid (TFC). The factors which influenced most TPC TFC RSL, ultra-turrax speed. Twelve acids (rosmarinic acid, p-coumaric...

Abstract Funding Acknowledgements Type of funding sources: None. Background Present guidelines endorse complete removal cardiovascular-implantable electronic devices (pacemakers/defibrillators), including extraction intracardiac electrodes, not only for systemic infections but also localized "pocket infections." Objectives We evaluated the efficacy delivering "Continuous, In-situ-Targeted, ultr...

• A comparison of three different analytical proposals for the analysis PPCPs in sewage sludge was presented. clean-up step essentially preceded by extraction technique UAE. direct injection UAE extract with no prior LC-MS/MS provided best performance. The resulting method validated and successfully applied dewatered digested samples. Acetaminophen reported concentrations above 1,000 ng g −1. T...