A. Introduction B. Optical and Spectrophotometric Detectors 1. Differential refractometry 2. Ultraviolet spectrophotometry 3. Fluorescence detection 4. Infrared spectrophotometric detectors 5. Spectrophotometric detection with post-column chemical reaction C. Some Miscellaneous Detection Systems 1. The mass spectrometer as an HPLC detector 2. Radioactivity detectors 3. Density, electrochemical ...

The process of dehydration of glucose to 5-hydroxymethylfurfural (HMF), using caprolactam-lithium chloride (CPL/LiCl) as a solvent, was investigated. Dual-wavelength ultraviolet spectrophotometry provides a new approach for the determination of glucose conversion rate and yield of HMF. Experiments were performed to demonstrate the accuracy and precision of this method. Various reaction paramete...

The emergence and future of mammalian synthetic biology depends on technologies for orchestrating and custom tailoring complementary gene expression and signaling processes in a predictable manner. Here, we demonstrate for the first time multi-chromatic expression control in mammalian cells by differentially inducing up to three genes in a single cell culture in response to light of different w...

The N2 Carroll-Yoshino (CY) c94 2 X (0,0) and (0,1) Rydberg bands between 95 and 99 nm were reported to be the most prominent EUV emission features in Voyager 1 ultraviolet spectrometer (UVS) airglow spectra from Titan’s atmosphere. Although c94 is strongly excited by photoelectron impact, the (0,0) band is optically thick near peak production, so a multiple-scattering model is employed to calc...

The objective of the study was to investigate the in vitro antioxidation activity of lycium barbarum polysaccharides (LBP). Ultraviolet spectrophotometry was adopted to determine the capability of LBP to clear superoxide anions, hydroxyl radicals, DPPH free radicals and ABTS free radicals. The result showed that the law for LBP to clear superoxide anions, hydroxyl radicals and DPPH free radical...

Drug impurities are now seen as a major threat to drug safety around the world, especially when it comes carcinogenic impurities. Here, we present first spectrophotometric approach for quantification of lignocaine and fluorescein in presence 2,6-xylidine, lignocaine’s impurity. The depends on overcoming unresolved bands through data processing strategy employing 10 affordable, simple, sensitive...

Long-term outdoor experiments were conducted to investigate the effects of elevated ultraviolet-B (UV-B, 280-320 nm) radiation on secondary metabolites (phenolics and terpenoids) and the main soluble sugars (sucrose, raffinose and glucose) in the bark of silver birch (Betula pendula Roth) saplings. Saplings were exposed to a constant 50% increase in erythemal UV irradiance (UV-B(CIE); based on ...

Aim to develop a method for standardization of licorice herb.
 Material and methods. The material the study was herb harvested in Alekseevka settlement Samara Region (2021), Tatarskaya Kargala village Sakmarskiy area Orenburg (2017), Derzhavinsk city Republic Kazakhstan (2018), Bol'shaya Chernigovka Bol'shchernigovsk (2019), commercial sample root raw from AO "Krasnogorskleksredstva". In a...

BACKGROUND Photo(chemo)therapy is widely used to treat psoriasis, the pathogenesis of which might be caused by an imbalance of Th17 cells/regulatory T cells (Treg). In the present study, we evaluated the effects of photo(chemo)therapy on the Th17/Treg balance and Treg function. METHODS Peripheral blood was obtained from psoriasis patients treated with bath-psoralen ultraviolet A (UVA, n = 50)...

UNLABELLED • PREMISE OF THE STUDY Although ultraviolet radiation (UV) is known to have negative effects on plant growth, there has been no direct evidence that plants growing at higher elevations are more severely affected by ultraviolet-B (UV-B) radiation, which is known to increase with elevation. We examined damage to DNA, a primary target of UV-B, in the widespread species Polygonum sacha...