نتایج جستجو برای: umbilical cord matrix mesenchymal stem cells

تعداد نتایج: 1888600  

2017
Ghmkin Hassan Issam Kasem Chadi Soukkarieh Majd Aljamali

Background and Objectives Mesenchymal stem cells (MSCs) are multipotent stem cells that can be isolated from umbilical cords and are therapeutically used because of their ability to differentiate into various types of cells, in addition to their immunosuppressive and anti-inflammatory properties. Fetal bovine serum (FBS), considered as the standard additive when isolating and culturing MSCs, ha...

Journal: :Revista brasileira de cirurgia cardiovascular : orgao oficial da Sociedade Brasileira de Cirurgia Cardiovascular 2008
Rosa Sayoko Kawasaki-Oyama Domingo Marcolino Braile Heloisa Cristina Caldas João Carlos Ferreira Leal Eny Maria Goloni-Bertollo Erika Cristina Pavarino-Bertelli Mário Abbud Filho Izaura Dos Santos

OBJECTIVES Implantation of cell separation and mesenchymal stem cell culture techniques from human umbilical cord blood with and without using the Ficoll-Paque gradient density method (d=1.077 g/ml). METHODS Ten samples of the umbilical cord blood obtained from full-term deliveries were submitted to two different procedures of mesenchymal stem cell culture: a) Method without the Ficoll-Paque ...

Journal: :iranian journal of immunology 0
zeynab aliyari stem cell research center forogh alemi stem cell research center balal brazvan tissue engineering research group, advanced research school, tabriz university of medical sciences, tabriz, iran hamid tayefi nasrabadi tissue engineering research group, advanced research school, tabriz university of medical sciences, tabriz, iran hojjatollah nozad charoudeh stem cell research center

background: umbilical cord blood (ucb) is an alternative source of hematopoietic stem cell transplantation (hsct), used in leukemia treatment. cd26+ cells, a fraction of cd34 positive cells, are a major population of ucb cells which negatively regulate the in vivo homing and engraftment of hscs. cd26 is highly expressed in various cells such as hscs, immune cells, fibroblasts, and epithelial ce...

Journal: :iranian journal of blood and cancer 0
mehrabi habibabadi h amiri f moslemi e habibi roudkenar m jalili m

background: mesenchymal stem cells (mscs) are ideal cells for cell and gene therapy. however, the low survival of mscs after transplantation has limited their applications. we aimed to evaluate the expression of cytoprotective genes including nqo1, txnrd1, ho-1, gclc following the over expression of nrf2 in umbilical cord-derived mscs (uc-mscs). methods: 3-5 passages of uc-mscs were cultured. r...

2013
Ying Hu Jun Liang Hongping Cui Xinmei Wang Hua Rong Bin Shao Hao Cui

Human Wharton's jelly mesenchymal stem cells were isolated from fetal umbilical cord. Cells were cultured in serum-free neural stem cell-conditioned medium or neural stem cell-conditioned medium supplemented with Dkk-1, a Wnt/β catenin pathway antagonist, and LeftyA, a Nodal signaling pathway antagonist to induce differentiation into retinal progenitor cells. Inverted microscopy showed that aft...

2013
Wuyi Kong Mu Nuo Xiao Ping Zhu Xiu Juan Han Lihua Luo Xian Wang

We found a group of non-platelet RNA-containing particles (NPRCP) in human umbilical cord blood. To understand the origin, characterization and differentiation of NPRCP, we examined cord blood-isolated NPRCP in vitro. The NPRCP range in size from < 1 to 5 μm, have a thin bilayer membrane and various morphological features, contain short RNA and microRNA and express octamer-binding transcription...

Journal: :Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie 2016
Diana Maria Anastasiu Ada Cean Maria Florina Bojin Adrian Gluhovschi Carmen Panaitescu Virgil Păunescu Gabriela Tănăsie

In recent years, identification of new sources of adult stem cells developed rapidly, pursuing to find easily available tissues, which will give rise to homogenous stem cells populations. Up to present, bone marrow-derived mesenchymal stem cells (BM-MSCs) are unanimously considered to fulfill the criteria for being used in clinical settings, but adipose stem cells, placental and umbilical cord ...

2014
Cristiana Leite N. Tatiana Silva Sandrine Mendes Andreia Ribeiro Joana Paes de Faria Tânia Lourenço Francisco dos Santos Pedro Z. Andrade Carla M. P. Cardoso Margarida Vieira Artur Paiva Cláudia L. da Silva Joaquim M. S. Cabral João B. Relvas Mário Grãos

Mesenchymal stem cells (MSCs) are viewed as safe, readily available and promising adult stem cells, which are currently used in several clinical trials. Additionally, their soluble-factor secretion and multi-lineage differentiation capacities place MSCs in the forefront of stem cell types with expected near-future clinical applications. In the present work MSCs were isolated from the umbilical ...

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