نتایج جستجو برای: universal primers
تعداد نتایج: 133448 فیلتر نتایج به سال:
We developed and evaluated a two-step PCR-based assay with universal primers and genus- or species-specific primers for the detection of the most prevalent bacterial etiologies of otitis media with effusion (OME) in children from Lebanese hospitals. These etiologies included Haemophilus, Streptococcus, and Moraxella (Branhamella) catarrhalis, which were detected in middle-ear effusion (MEE) sam...
OBJECTIVES In order to develop a multiplex RT-PCR assay using the GeXP analyser for the simultaneous detection of four different NA serotypes of H5-subtype AIVs, effective to control and reduce H5 subtype of avian influenza outbreak. DESIGN Six pairs of primers were designed using conserved and specific sequences of the AIV subtypes H5, N1, N2, N6 and N8 in GenBank. Each gene-specific primer ...
the dry bubble disease, caused by lecanicillium fungicola, is an important fungal disease of white button mushroom in iranian mushroom production farms. twenty-three isolates of the pathogen collected in iran and identified as l. fungicola var. fungicola, were compared for genetic polymorphism, diversity in growth rate and virulence. ten universal rice primers (urp) were used to evaluate the ge...
The spatiotemporal distribution of fish larvae and eggs is fundamental for their reproduction recruitment in aquatic ecosystems. Here, a metabarcoding strategy was employed as an alternative to conventional ichthyoplankton survey, which requires considerable amount time, labor, cost. First, piscine-specific universal primer set (FishU) designed amplify the region, flanking highly conserved mito...
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