BACKGROUND Gene expression profiling of the salivary supernatant is emerging as a new and important source of real-time, systemic, biological information. However, existing technologies prevent RNA extraction of small quantities found in neonatal salivary supernatant. OBJECTIVE The aim of this study was to develop techniques to enhance extraction of cell-free RNA from neonatal salivary supern...

Shrews (family Soricidae ) are tiny mole-shaped mammals belong to the order Eulipotyphla . The main objective of this study is screen wild specimens shrews for specific RNA viruses cause hemorrhagic fevers. Wild were collected from rural areas in Kingdom Saudi Arabia. Collection carried out traps then identified using classical morphological keys. Specimens dissected extraction single stranded ...

Objective and Material and Methods This study aimed to compare the quantity and quality of human DNA extracted from saliva that was fresh or frozen for three, six and twelve months using five different DNA extraction protocols: protocol 1 - Oragene™ commercial kit, protocol 2 - QIAamp DNA mini kit, protocol 3 - DNA extraction using ammonium acetate, protocol 4 - Instagene™ Matrix and protocol 5...

Dermatophilus congolensis, the aetiological agent of dermatophilosis, is a pleomorphic, Gram-positive actinomycete, which infects animals and humans. Often, there is a wrong diagnosis of the infection in animals because of the close resemblance of the organism with other members of the family Actinomycetaceae. In this study, molecular tools were applied to suspected isolates of D. congolensis o...

objective(s): the aim of present research was to develop matrix-mini-tablets of lornoxicam filled in capsule for targeting early morning peak symptoms of rheumatoid arthritis. materials and methods:matrix-mini-tablets of lornoxicam were prepared by direct compression method using microsomal enzyme dependent and ph-sensitive polymers which were further filled into an empty hpmc capsule. to asses...

The Latvian population of the Forest Dormouse is small and isolated, situated far from main distribution range this species. located in south-eastern region Latvia, close to Belarusian border. Sixty-three nest-boxes were throughout area captured forest dormouse individuals sampled for genetic analysis. Animal age, sex, coordinates place capture recorded. DNA was extracted 120 using QIAamp Mini ...

DNA methylation analyses usually require a preceding bisulfite conversion of the DNA. The choice of an appropriate kit for a specific application should be based on the specific performance requirements with regard to the respective sample material. In this study, the performance of nine kits was evaluated: EpiTect Fast FFPE Bisulfite Kit, EpiTect Bisulfite Kit, EpiTect Fast DNA Bisulfite Kit (...