Emerging evidence that Enterovirus A 71 (EV-A71) infection closely related to apoptosis. The ZVAD is a caspase inhibitor can prevent aims of this project were evaluate the mechanism inhibited EV-A71 virus and provide experiment basis for finding new antiviral drugs. In study, after treated with in infected Vero cells, viral replication was reduced, cell viability higher than group. Additionally...

background: it was shown that olive leave extract has antifungal, antibacterial and antiviral activities. the effects of ole on herpes simplex virus-1 (hsv-1) have not been systematically investigated yet. the aim of this study was to examine the in vitro effect of olive leaf hydroalcoholic extract (olhe) on hsv-1.   methods: virucidal effect and viral replication in vero cell line were studied...

Rose bengal dye is thought to highlight corneal lesions induced by herpes simplex virus type 1 (HSV-1) by virtue of its binding to dead or dying HSV-1-infected epithelial cells. However, whether rose bengal binds specifically to damaged versus normal corneal epithelial cells is unclear. To determine the binding properties of rose bengal, the authors compared binding of the dye to HSV-1-infected...

Virus inducible elements (IE) in promoters of mouse alpha-interferon and human beta 1-interferon genes contain multiple copies of the hexanucleotide sequence AGT-GAA or its variants which are also found in the interferon-stimulated response element of genes transcriptionally induced by interferon. We have examined the similarities between virus and interferon induction of gene expression and th...

In the present study, investigations were carried out to screen the anticancer activities of fenugreek seed oil against cancer cell lines (HEp-2, MCF-7, WISH cells), and a normal cell line (Vero cells). Cytotoxicity was assessed with MTT and NRU assays, and cellular morphological alterations were studied using phase contrast light microscopy. All cells were exposed toi 10-1000 μg/ml of fenugree...

Vero cells and Madin Darby Canin Kidney cells were exposed to Salmonella typhimurium sub spp. enteritis to study the interaction and invasion. The monolayer cell line bottles were selected and sub cultured. The sub cultured bottles were incubated at 37°C in 5% CO atmosphere for the formation of monolayer. 2 After the complete confluence of cells, the bottles were selected for the inoculation of...

The nature of the initial interaction of dengue virus with target cells and the extent to which this interaction defines tropism are unknown. Infection of some cells may involve antidengue antibody-mediated immune adherence to cells bearing immunoglobulin Fc receptors; however, this mechanism does not explain primary infection or the infection of cells without Fc receptors. We hypothesized that...

background: the aim of the study was to provide a point of reference to study the neospora caninum infections in china. methods: genome dna was extracted from the brains of aborted fetuses and specific pcr was performed with n. caninum nc5-targeted specific primers. fetal bovine brain tissues were homogenized and continuously cultured in vero cells with double antibodies. the medium was replace...

Dengue viruses are the most important arthropod-borne viruses in terms of morbidity and mortality in the world. Since there is no dengue vaccine available for human use, we have set out to investigate the use of chloroquine as an antiviral drug against dengue. Chloroquine, an amine acidotropic drug known to affect intracellular exocytic pathways by increasing endosomal pH, was used in the in vi...