N-Bromoacetylethanolamine phosphate, prepared by the bromoacetylation of ethanolamine phosphate, has been tested as an active site-specilic reagent for rabbit and rat muscle fructose diphosphate aldolases. The reagent inactivates both enzymes, and inactivation is prevented by substrates or competitive inhibitors. Loss of activity is pseudo-first order until the later stages of inactivation, and...

A novel immobilized dehydrocondensing reagent comprised of a triazine-type dehydrocondensing reagent itself in a polymerized form was synthesized by copolymerization between tetra(ethylene glycol) bis(dichlorotriazinyl) ether and tris(2-aminoethyl)amine.

A new spray reagent with copper, ammonium molybdate, and sulphuric acid for the detection of phospholipids on thin-layer chromatograms is described. The specificity and sensitivity of the spray reagent is also shown.

A novel proton acceptor coupling reagent shows superiority to those described previously. The oxygen in the carbocation moiety confers more solubility to the reagent. Furthermore, it enhances coupling yields and decreases racemization, allowing the use of 1 equiv of base.

In connection with the study of the state of the calcium of the blood, we desired a rapid method for determining serum proteins. Wu (1) and Wu and Ling (2) have published a method for the determination of plasma proteins based on the color developed with Folin’s phenol reagent. The method as described by these authors is not very satisfactory due to the formation of turbid precipitates and the ...

The use of protein reagents which exhibit sensitivity to changes in molecular environment has provided a means of examining certain properties of enzymes during interaction with their substrates (1). The recently reported 2-hydroxy-5nitrobenzyl bromide provided one such reagent, the absorption spectrum of which showed sensitivity to environmental changes by virtue of the p-nitrophenolic groupin...

BACKGROUND The favorable properties of lanthanide chelates compared with conventional fluorescent probes have attracted considerable interest. A Eu(3+) chelator, 4,7-bis(chlorosulfophenyl)-1, 10-phenanthroline-2,9-dicarboxylic acid (BCPDA), has been synthesized previously. METHODS We here describe immunoassay, immunohistochemistry, and microarray applications of a new streptavidin-based unive...

Our objective was to investigate the protective effect of Lawesson's reagent, an H2S donor, against alendronate (ALD)-induced gastric damage in rats. Rats were pretreated with saline or Lawesson's reagent (3, 9, or 27 µmol/kg, po) once daily for 4 days. After 30 min, gastric damage was induced by ALD (30 mg/kg) administration by gavage. On the last day of treatment, the animals were killed 4 h ...