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objective: this study was designed to evaluate the effects of vitrification and in vitro culture of human ovarian tissue on the expression of oocytic and follicular cell-related genes. materials and methods: in this comparative study, ovarian tissue samples were obtained from eight transsexual women. samples were cut into small fragments and were then assigned to vitrified and non-vitrified gro...

This review considers the relationship of in vitro gamete maturation and embryo culture to the future development of animal biotechnology. The areas reviewed are oocyte maturation in vitro and embryo culture and their importance for successful in vitro embryo production. The rapidly developing area of spermatogonial cell transplantation and culture is also reviewed. The scientific milestones le...

Background Mesenchyme stem cells (MSCs) are one of the widely used sources in tissue engineering and efficacy of cultural environment. In this survey, rat stem cells are used as coculture. Owing to main obstacle of embryo culture in the lab medium, the survival of embryo in lab is low in comparison with that of normal conditions. The purpose of this research is the survey of fertilization and c...

Light is important for the production of metabolites by plant cell culture. It is therefore necessary to evaluate the light conditions with respect to exposure time. Both enhancement and inhibition have been observed depending on exposure time and species. Callus culture of D. nervosa was established and effect of light and passage on cardiac glycoside production was studied. In vitro cultures ...

A reliable method is described for rapid proliferation of axillary shoots from nodal segment of mature field-grown aromatic and medicinal plant Vitex negundo through in vitro culture. Of different concentrations of N6-benzyladenine (BA) evaluated as supplements to Murahige and Skoog (MS) medium, BA at 1mg/l was effective in enhancing bud break and shoot regeneration. Response of explants was al...

introduction: standard concentrations of antibiotics in culture media are thought to have no detectable toxic effects on the cultured cells. however, since antibiotics are biologically active substances, the possibility that they interfere to some extent with cellular processes occurring in the cultured cells can not always be totally excluded. this study, therefore, was conducted to assess whe...

a reliable method is described for rapid proliferation of axillary shoots from nodal segment of mature field-grown aromatic and medicinal plant vitex negundo through in vitro culture. of different concentrations of n6-benzyladenine (ba) evaluated as supplements to murahige and skoog (ms) medium, ba at 1mg/l was effective in enhancing bud break and shoot regeneration. response of explants was al...

I n vitro rumen experiments have been conducted for at least 40 years, and many research problems have been examined successfully by this approach. The simplest sub-division of in vitro rumen systems is into batchand continuous-culture types. The batch-culture approach involves the provision of an initial supply only of substrate, which results in a culture in which the concentration of substra...