Monocyte-derived macrophages (MDM) can polarize into different subsets depending on the environment and the activation signal to which they are submitted. Differentiation into macrophages allows HIV-1 strains to infect cells of the monocytic lineage. In this study, we show that culture of monocytes with a combination of IL-12 and IL-18 led to macrophage differentiation that was resistant to HIV...

Considering that a set of three genes is sufficient for the replication of most retroviruses, the human and simian immunodeficiency viruses (HIV and SIV) exhibit a surprising degree of genomic complexity. HIV-1 contains no less than six open reading frames in addition to the prototypic gag, pol, and env coding sequences (see Figure 1). Two of these supplementary genes, tat and rev, are absolute...

We evaluated cellular metabolism profiles of HIV-1 and HIV-2 infected primary human monocyte-derived macrophages (MDMs). First, HIV-2 GL-AN displays faster production kinetics and greater amounts of virus as compared to HIV-1s: YU-2, 89.6 and JR-CSF. Second, quantitative LC-MS/MS metabolomics analysis demonstrates very similar metabolic profiles in glycolysis and TCA cycle metabolic intermediat...

SAMHD1 restricts the replication of HIV-1 and other retroviruses in human myeloid and resting CD4(+) T cells and that is counteracted in SIV and HIV-2 by the Vpx accessory protein. The protein is a phosphohydrolase that lowers the concentration of deoxynucleoside triphosphates (dNTP), blocking reverse transcription of the viral RNA genome. Polymorphisms in the gene encoding SAMHD1 are associate...

Like other HIV-1 auxiliary proteins, Vpr is conserved within all the human (HIV-1, HIV-2) and simian (SIV) immunodeficiency viruses. However, Vpr and homologous HIV-2, and SIV Vpx are the only viral auxiliary proteins specifically incorporated into virus particles through direct interaction with the Gag precursor, indicating that this presence in the core of the mature virions is mainly require...

Methods Nineteen subjects (n=19; 8 male, 11 female; age 22.42 ± 3.15 years; body mass: 68.95 ± 12.70 kg; BMI: 23.86 ± 2.85; ht: 168.7 cm) volunteered to participate in the study. All test subjects completed a health history and medical questionnaire, as well as an informed consent form, prior to participation. Participants completed pre and post-testing, consisting of the following: Profile of ...