Background and Aims: Since the produced recombinant proteins by molecular genetics techniques commonly have some limitations in the application, chimeric protein are introduced. Chimeric proteins have found widespread application for the study of protein folding, structure stability, function and immunogenisity. Methods: According to the known Immunomodulatory effect and structure of HSP70 mole...

For tracking accuracy of moving target in wide-area video surveillance system is not high, a tracking and recognition algorithm based on multi-source moving target is proposed. The fusion algorithm preliminarily determines candidate moving target by frame difference algorithm, and then matches candidate target with primary template by SIFT algorithm and treats the matching result as initial pos...

Enzyme-free cloning (EFC) can rapidly produce an in-frame fusion gene with multiple fragments. To practically apply EFC, we investigated the extent and sequence of complementary staggered overhangs necessary to direct self-assembly of multiple fragments as well as a size limitation of the constructed DNA molecule. Six-base pair overhangs with 50% GC content were sufficient to direct self-assemb...

Zinacantec Maya weaving tools and apprenticeship practices contain an implicit theory of cognitive development that corresponds to Piaget and Inhelder’s explicit theory of cognitive development [The Child’s Conception of Space, London, Routledge and Kegan Paul, 1956]. A set of preoperational and concrete operational spatial problems from the weaving domain provided empirical support for the eth...

purpose : to determine the effect of carpet weaving on refractive errors methods : in this cross sectional study, carpet weavers and non-weavers in the normal population of mashhad were regarded as exposed and non-exposed groups, respectively. a carpet weaver was a person who wove carpets 7 hours a day for at least 2 years. the non-weavers group was selected from the population of mashhad throu...

We present an angiofibroma of soft tissue with the karyotype 46,XY,t(4;5)(q24;q31),t(5;8;17)(p15;q13;q21)[8]/46,XY,t(1;14)(p31;q32)[2]/46,XY[3]. RNA‑sequencing showed that the t(4;5)(q24;q31) resulted in recombination of the genes TBCK on 4q24 and P4HA2 on 5q31.1 with generation of an in‑frame TBCK‑P4HA2 and the reciprocal but out‑of‑frame P4HA2‑TBCK fusion transcripts. The putative TBCK‑P4HA2 ...

Plural unit selection and fusion is a successful method for concatenative synthesis. Yet its unit fusion algorithm is simple and requires improvement. Previous research on unit fusion is mainly involved in boundary smoothing and not quite suitable for the application mentioned above. Therefore, a high-quality unit fusion method is proposed in this paper. More accurate pitch frame alignment and ...

Mulhern Automated proof assistants provide few facilities for incremental development. Generally, if the underlying structures on which a proof is based are modified, the developer must redo much of the proof. Yet incremental development is really the most natural approach for proofs of programming language properties [5, 12]. We propose " proof weaving " , a technique that allows a proof devel...