3-Hydroxy-3-methylglutaryl coenzyme A reductase (HMGR, EC1.1.1.34), the key enzyme in isoprenoid biosynthesis, was purified from microsomes of potato tuber tissue, and a polyclonal antibody and two monoclonal antibodies against the purified enzyme were prepared. HMGR protein content was measured by immunotitration and radioimmunoassay using these antibodies. HMGR activity was very low in the fr...

The effects of row proportions and nutrient management was studied in potato (Solanum tubersoum L.) + Indian mustard [Brassica juncea (L.) Czernj.] intercropping system during 2014–15 2015–16 at Amar Singh College Farm, Lakhaoti (Bulandshahr), Uttar Pradesh. field experiment laid out a split-split plot design with 3 replications. main treatments consisted varieties, viz. ‘Kufri Pukhraj’, Anand’...

Abstract Background Gloriosa superba L. belongs to Colchicaceae, which is an important medicinal plant containing high-priced alkaloid colchicines and other potent phytochemicals. Due its extensive importance at the industrial level, this overexploited. Moreover, indiscriminate harvesting for raw material leads a decline in population of natural environment. Thus, present study deals with optim...

Venom neutralizing activity of crude root extract of Corallocarpus epigaeus was determined by in vivo procedure recommended by WHO. Neutralization assays for antivenom is necessary to replace the in vitro neutralization assay. Albino mice were used to estimate venom neutralizing activity of tuber extract. Corallocarpus epigaues tuber extract preparations exhibit immunomodulatory properties. Thi...

To understand the meaning of blending crude drugs in Chinese medicinal prescriptions, the influence of Saussurea root on the pharmacological action of Corydalis tuber was examined. Saussurea root increased the depression of acetylcholine-induced contraction caused by the hot water extract solution of Corydalis tuber in mouse ileum at low dosage, which showed no direct influence on acetylcholine...

Multiple disease resistance is an aim of many plant breeding programs. Previously, novel somatic cell selection was used to generate potato variants of "Russet Burbank" with resistance to common scab caused by infection with an actinomycete pathogen. Coexpression of resistance to powdery scab caused by a protozoan pathogen was subsequently shown. This study sought to define whether this resista...

1 We used a combination of laboratory and field experiments to test the hypothesis that the burial depth of Potamogeton pectinatus tubers will vary with local sediment type and swan predation pressure. 2 In the field, mortality due to predation by swans decreased linearly with burial depth (from 100% at the surface to 55% at 225 mm depth) and with sediment clay content. Average tuber size showe...

Although cooked potato tuber texture is an important trait that influences consumer preference, a detailed understanding of tuber textural properties at the molecular level is lacking. Previous work has identified tuber pectin methyl esterase activity (PME) as a potential factor impacting on textural properties. In this study, tuber PME isoform and gene expression profiles have been determined ...

The lipid biopolymer suberin plays a major role as a barrier both at plant-environment interfaces and in internal tissues, restricting water and nutrient transport. In potato (Solanum tuberosum), tuber integrity is dependent on suberized periderm. Using microarray analyses, we identified ABCG1, encoding an ABC transporter, as a gene responsive to the pathogen-associated molecular pattern Pep-13...