We aimed to use a novel standardized whole-blood stimulation system evaluate differences in the functional immune reconstitution patients early after allogeneic haematopoietic cell transplantation (HCT). Between April and September 2018, 30 undergoing HCT had whole blood samples collected around day ?21 (day 0 being of infusion) +28. Whole was transferred TruCulture assays comprising prefilled ...

BACKGROUND Rapid identification of the etiological agent in bloodstream infections is of vital importance for the early administration of the most appropriate antibiotic therapy. Molecular methods may offer an advantage to current culture-based microbiological diagnosis. The goal of this study was to evaluate the performance of IRIDICA, a platform based on universal genetic amplification follow...

THP-1 cells are widely applied to mimic monocytes in cell culture models. In this study, we compared the cytokine release from THP-1, peripheral blood mononuclear cells (PBMC), monocytes, or whole blood after stimulation with lipopolysaccharide (LPS) and investigated the consequences of different cytokine profiles on human umbilical vein endothelial cell (HUVEC) activation. While Pseudomonas ae...

The present study compares the effect of various lectins; phytohaemagglutinin (PHA), Pokeweed mitogen (PWM) and Concanavalin-A (Con A) on short term lymphocyte culture and morphology of chromosome in cattle and buffaloes. For the comparative studies, the whole blood culture from 5 cattle and 5 buffaloes in triplicate were set up by adding 3 different types of lectins. Results based on physical ...

Recent cancer research has demonstrated the existence of circulating tumor cells (CTCs) in cancer patient's blood. Once identified, CTC biomarkers will be invaluable tools for clinical diagnosis, prognosis and treatment. In this review, we propose ex vivo culture as a rational strategy for large scale amplification of the limited numbers of CTCs from a patient sample, to derive enough CTCs for ...

BACKGROUND Cryptococcus neoformans is the most common cause of adult meningitis in sub-Saharan Africa. The cryptococcal antigen (CRAG) lateral flow assay (LFA) has simplified diagnosis as a point-of-care test approved for serum or cerebrospinal fluid (CSF). We evaluated the accuracy of the CRAG LFA using fingerstick whole blood compared with serum/plasma and CSF for diagnosing meningitis. MET...

Researchers often purify DNA from blood samples prior to performing PCR because it is believed that blood constituents and the reagents commonly used to preserve blood samples (e.g., anticoagulants) interfere with PCR. In order to save the time and expense required for template purification, several methods have been reported for direct PCR of blood samples. These include microwave irradiation,...

The reference method for the diagnosis of bloodstream infections is blood culture followed by biochemical identification and antibiotic susceptibility testing of the isolated pathogen. This process requires 48 to 72 hours. The rapid administration of the most appropriate antimicrobial treatment is crucial for the survival of septic patients; therefore, a rapid method that enables diagnosis dire...

The purpose of this paper 2 is to present the results of experimental work on the preservation of whole blood in vivo and in vitro carried out over a period of several years. Certain standards of preservation in vitro (the osmotic resistance) and in vivo (the serum bilirubin variations) have been carried out extensively in the early as well as in the late experiments, thus affording a means of ...