Of 38 pure cultures of microorganisms tested, only one, Pseudomonas stutzeri, was capable of forming dimethylnitrosamine from dimethylamine and nitrite during growth. Resting cells of P. stutzeri, Cryptococcus terreus, Escherichia coli, and Xanthomonas campestris formed dimethylnitrosamine, although no nitrosamine was found in growing cultures of the latter three organisms. No nitrosamine was p...

The Gram-negative bacterial genus Xanthomonas includes numerous infra-specific taxa known as pathovars, which are defined primarily on host range and disease symptoms. With the advent of molecular sequence data, many pathovars have been transferred from X. campestris into other species to better harmonise taxonomy phylogeny. We performed whole-genome shotgun sequencing pathotype strains followi...

The oligopeptide-binding protein, OppA, binds and ushers oligopeptide substrates to the membrane-associated oligopeptide permease (Opp), a multi-component ABC-type transporter involved in the uptake of oligopeptides expressed by several bacterial species. In the present study, we report the cloning, purification, refolding and conformational analysis of a recombinant OppA protein derived from X...

In Xanthomonas citri, the bacterium that causes citrus canker, three ATP-binding cassette (ABC) transporters are known to be dedicated to the uptake of sulfur compounds. In this work, using functional, biophysical and structural methods, we showed that NrtT, a periplasmic component of the ABC transporter NrtCB, is an alkanesulfonate-binding protein and that the deletion of the nrtT gene affecte...

For modeling biological systems the already existing Petri Net Libraries were further developed with OpenModelica using the SimForge graphical user interface (GUI). The Petri Nets elements were wrapped into models for different reaction types to simplify the modeling process. Additionally, a database connection was implemented for integrating kinetic data. The application of this new Reaction L...

There is a world wide interest in searching for the safe and effective novel antibacterial compounds of plant origin for the control of plant pathogenic bacteria which is responsible for the great impact on the growth and productivity of agriculture crops. In this study an attempt was made to determine the in vitro antibacterial activity of sequentially extracted different solvent (dichlorometh...